A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo

被引：252

作者：

Coelho, Pedro S. ^{[1
]}

Wang, Z. Jane ^{[1
]}

Ener, Maraia E. ^{[1
]}

Baril, Stefanie A. ^{[2
]}

Kannan, Arvind ^{[1
]}

Arnold, Frances H. ^{[1
]}

Brustad, Eric M. ^{[2
,3
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Univ N Carolina, Dept Chem, Chapel Hill, NC USA

[3] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA

来源：

NATURE CHEMICAL BIOLOGY | 2013年 / 9卷 / 08期

基金：

美国国家卫生研究院;

关键词：

STEREOSELECTIVE CYCLOPROPANATION; HEME; SYSTEM;

D O I：

10.1038/nchembio.1278

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whole-cell catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a cytochrome 'P411' with unique serine-heme ligation that catalyzes efficient and selective olefin cyclopropanation in intact Escherichia coli cells. The mutation C400S in cytochrome P450(BM3) gives a signature ferrous CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting-state Fe-III-to-Fe-II reduction potential and substantially improves NAD(P) H-driven activity.

引用

页码：485 / U33

页数：6

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