Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled carbohydrate-binding module

被引：4

作者：

Ojima, Yoshihiro ^{[1
]}

Suparman, Asep ^{[1
]}

Minh Hong Nguyen ^{[1
]}

Sakka, Makiko ^{[2
]}

Sakka, Kazuo ^{[2
]}

Taya, Masahito ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn Sci, Div Chem Engn, Toyonaka, Osaka 5608531, Japan

[2] Mie Univ, Grad Sch Bioresources, Appl Microbiol Lab, Tsu, Mie 5148507, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 114卷

关键词：

Carbohydrate-binding module; Exopolysaccharide; Escherichia coli; Microcolony; LINKED LECTINSORBENT ASSAY; BACTERIAL BIOFILMS; MATRIX;

D O I：

10.1016/j.mimet.2015.05.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative evaluation of the EPShs. (C) 2015 Elsevier B.V. Al! rights reserved.

引用

页码：75 / 77

页数：3

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