Simultaneous determination of curcuminoids in Curcuma samples using high performance thin layer chromatography

被引:95
作者
Gupta, AP [1 ]
Gupta, MM [1 ]
Kumar, S [1 ]
机构
[1] Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India
关键词
D O I
10.1081/JLC-100101751
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid and simple high performance thin layer chromatographic method has been developed for the simultaneous quantitation of pharmacologically important diaryl heptanoids curcumin, demethoxy curcumin, and bis-demethoxy curcumin in Curcuma longa and C. amada. The assay combines the isolation and separation of curcuminoids on silica gel 60F(254) high performance thin layer chromatographic plates, followed by scanning of the spots at 366 nm using a UV detection mode.
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页码:1561 / 1569
页数:9
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