Isolation of bovine corneal keratan sulfate and its growth factor and morphogen binding

被引:44
作者
Weyers, Amanda [1 ]
Yang, Bo [1 ]
Solakyildirim, Kemal [1 ]
Yee, Vienna [1 ]
Li, Lingyun [1 ]
Zhang, Fuming [2 ]
Linhardt, Robert J. [1 ,2 ,3 ,4 ]
机构
[1] Rensselaer Polytech Inst, Dept Chem & Chem Biol, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
[2] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
[3] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Biol, Troy, NY 12180 USA
[4] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Dept Biomed Engn, Troy, NY 12180 USA
基金
美国国家卫生研究院;
关键词
cornea; fibroblast growth factor; interaction; keratan sulfate; sonic hedgehog; SONIC HEDGEHOG EXPRESSION; II DIGESTION; OLIGOSACCHARIDES; GLYCOSAMINOGLYCANS; HEPARINASE; PROTEOGLYCANS; BIOSYNTHESIS; LUMICAN; DECORIN; CLONING;
D O I
10.1111/febs.12165
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Keratan sulfate (KS) is an important glycosaminoglycan that is found in cartilage, reproductive tissues, and neural tissues. Corneal KS glycosaminoglycan is found N-linked to lumican, keratocan and mimecan proteoglycans, and has been widely studied by investigators interested in corneal development and diseases. Recently, the availability of corneal KS has become severely limited, owing to restrictions on the shipment of bovine central nervous system byproducts across international borders in an effort to prevent additional cases of mad cow disease. We report a simple method for the purification of multi-milligram quantities of bovine corneal KS, and characterize its structural properties. We also examined its protein-binding properties, and discovered that corneal KS bound with high affinity to fibroblast growth factor-2 and sonic hedgehog, a growth factor and a morphogen involved in corneal development and healing.
引用
收藏
页码:2285 / 2293
页数:9
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