Application Values of Cu(II) Coordination Polymer on Osteoarthritis Therapy by Inhibiting Inflammatory Cytokines Releasing

In this study, a new low dimensional Cu(II)-based coordination polymer with the chemical composition of {[Cu-3(L)(2)(phen)(H2O)(8)]center dot 4H(2)O} n (1, H3L = 5-(3 ',5 '-dicarboxyphenyl) nicotinic acid; phen = phenanthroline) have been synthesized via reaction of Cu(NO3)(2)center dot 3H(2)O with the H3L ligand in the presence of the N-donor ligand phen under the hydrothermal reaction conditions. Its application values on the osteoarthritis were explored and the mechanism was explored as well. Firstly, the levels of inflammatory cytokines released during osteoarthritis after compound treatment was measured with indicated enzyme linked immunosorbent assay (ELISA) detection kit. In addition to this, the real time Reverse Transcription Polymerase Chain Reaction (RT-PCR) was carried out and the activation of the inflammatory signaling pathway was detected.