An isolation procedure for arachidonic acid producing Mortierella species

被引:33
作者
Botha, A
Paul, I
Roux, C
Kock, JLF
Coetzee, DJ
Strauss, T
Maree, C
机构
[1] Univ Orange Free State, Dept Microbiol & Biochem, ZA-9301 Bloemfontein, South Africa
[2] Natl Collect Fungi, Biosyst Div, Plant Protect Res Inst, Pretoria, South Africa
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1999年 / 75卷 / 03期
关键词
arachidonic acid; isolation; Mortierella; soil;
D O I
10.1023/A:1001848709005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Malt extract agar and an incubation temperature of 5 degrees C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.
引用
收藏
页码:253 / 256
页数:4
相关论文
共 28 条
[1]  
AMANO N, 1992, MYCOTAXON, V44, P257
[2]   EICOSAPENTAENOIC ACID (EPA) PRODUCTION FROM MICROORGANISMS - A REVIEW [J].
BAJPAI, P ;
BAJPAI, PK .
JOURNAL OF BIOTECHNOLOGY, 1993, 30 (02) :161-183
[3]   ARACHIDONIC-ACID PRODUCTION BY FUNGI [J].
BAJPAI, PK ;
BAJPAI, P ;
WARD, OP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) :1255-1258
[4]  
BENNY G L, 1975, Aliso, V8, P301
[5]   OBSERVATIONS ON THAMNIDIACEAE .7. HELICOSTYLUM AND A NEW GENUS KIRKIA [J].
BENNY, GL .
MYCOLOGIA, 1995, 87 (02) :253-264
[6]  
BROOKS F. T., 1923, Transactions of the British Mycological Society, V8, P113
[7]   RAPID METHOD FOR THE DETERMINATION OF FATTY-ACID PROFILES FROM FATS AND OILS USING TRIMETHYLSULFONIUM HYDROXIDE FOR TRANS-ESTERIFICATION [J].
BUTTE, W .
JOURNAL OF CHROMATOGRAPHY, 1983, 261 (01) :142-145
[8]   ROOM-TEMPERATURE ISOLATIONS CAN BIAS AGAINST SELECTION OF LOW-TEMPERATURE MICROFUNGI IN TEMPERATE FOREST SOILS [J].
CARREIRO, MM ;
KOSKE, RE .
MYCOLOGIA, 1992, 84 (06) :886-900
[9]  
DOMSCH KH, 1980, COMPENIUM SOIL FUNGI
[10]   VERTICAL DISTRIBUTION OF FUNGI IN ZULULAND SOILS [J].
EICKER, A .
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1970, 55 :45-&