Improved DNA Clamps by Stacking to Adjacent Nucleobases

被引:4
作者
Fatthalla, Maha I. [1 ,2 ]
Pedersen, Erik B. [1 ]
机构
[1] Univ So Denmark, Dept Phys Chem & Pharm, Nucle Acid Ctr, DK-5230 Odense M, Denmark
[2] Helwan Univ, Fac Sci, Dept Chem, Cairo 11795, Egypt
关键词
Oligonucleotides; Locked nucleic acid (LNA); Molecular modeling; DNA Clamps; TRIPLE-HELIX FORMATION; LOCKED NUCLEIC-ACIDS; MOLECULAR BEACON STRATEGY; SINGLE-STRANDED-DNA; FORMING OLIGONUCLEOTIDES; ANTISENSE OLIGONUCLEOTIDES; MAMMALIAN-CELLS; TARGETING DNA; LNA; SEQUENCE;
D O I
10.1002/hlca.201200130
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Three or four aromatic rings interconnected by acetylene bridges form a stiff conjugated system with sufficient conformational freedom to make it useful to link together the two strands of a DNA clamp. Upon targeting a ssDNA, the conformational flexibility allows better stacking of the linker to the underlying non-planar base triplet in the formed triplex. This type of triplexes has a substantially higher thermal melting temperature which can be further improved by inserting locked nucleic acids (LNAs) in the Hoogsteen part of the clamp. An extremely high sensitivity to mismatches is observed in an octamer triplex when placed in the middle of the sequence.
引用
收藏
页码:1538 / 1547
页数:10
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