Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP

被引：263

作者：

Biteen, Julie S. ^{[1
]}

Thompson, Michael A. ^{[1
]}

Tselentis, Nicole K. ^{[1
]}

Bowman, Grant R. ^{[2
]}

Shapiro, Lucy ^{[2
]}

Moerner, W. E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 11期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1038/nmeth.1258

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.

引用

收藏

页码：947 / 949

页数：3

相关论文

共 15 条

[1] PROPOSED METHOD FOR MOLECULAR OPTICAL IMAGING [J].

BETZIG, E .

OPTICS LETTERS, 1995, 20 (03) :237-239

[2] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[3] The bacterial cytoskeleton:: In vivo dynamics of the actin-like protein Mbl of Bacillus subtilis [J].

Carballido-López, R ;

Errington, J .

DEVELOPMENTAL CELL, 2003, 4 (01) :19-28

[4] On/off blinking and switching behaviour of single molecules of green fluorescent protein [J].

Dickson, RM ;

Cubitt, AB ;

Tsien, RY ;

Moerner, WE .

NATURE, 1997, 388 (6640) :355-358

[5] MreB, the cell shape-determining bacterial actin homologue, co-ordinates cell wall morphogenesis in Caulobacter crescentus [J].

Figge, RM ;

Divakaruni, AV ;

Gober, JW .

MOLECULAR MICROBIOLOGY, 2004, 51 (05) :1321-1332

[6] Resolution of λ/10 in fluorescence microscopy using fast single molecule photo-switching [J].

Geisler, C. ;

Schoenle, A. ;

von Middendorff, C. ;

Bock, H. ;

Eggeling, C. ;

Egner, A. ;

Hell, S. W. .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2007, 88 (02) :223-226

[7] An actin-like gene can determine cell polarity in bacteria [J].

Gitai, Z ;

Dye, N ;

Shapiro, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (23) :8643-8648

[8] Ultra-high resolution imaging by fluorescence photoactivation localization microscopy [J].

Hess, Samuel T. ;

Girirajan, Thanu P. K. ;

Mason, Michael D. .

BIOPHYSICAL JOURNAL, 2006, 91 (11) :4258-4272

[9] Single molecules of the bacterial actin MreB undergo directed treadmilling motion in Caulobacter crescentus [J].

Kim, So Yeon ;

Gitai, Zemer ;

Kinkhabwala, Anika ;

Shapiro, Lucy ;

Moerner, W. E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (29) :10929-10934

[10] Single-molecule mountains yield nanoscale cell images [J].

Moerner, W. E. .

NATURE METHODS, 2006, 3 (10) :781-782