Super-resolution imaging in live Caulobacter crescentus cells using photoswitchable EYFP

被引：260
|
作者：
Biteen, Julie S. [1 ]
Thompson, Michael A. [1 ]
Tselentis, Nicole K. [1 ]
Bowman, Grant R. [2 ]
Shapiro, Lucy [2 ]
Moerner, W. E. [1 ]
机构：
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA
来源：
NATURE METHODS | 2008年 / 5卷 / 11期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
D O I：
10.1038/nmeth.1258
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The commonly used, monomeric EYFP enabled imaging of intracellular protein structures beyond the optical resolution limit ('super-resolution' imaging) in living cells. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. These studies demonstrated that EYFP is a useful emitter for in vivo super-resolution imaging.
引用
收藏
页码：947 / 949
页数：3
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