Lung microbiota across age and disease stage in cystic fibrosis

被引：300

作者：

Coburn, Bryan ^{[1
]}

Wang, Pauline W. ^{[2
]}

Caballero, Julio Diaz ^{[1
]}

Clark, Shawn T. ^{[3
]}

Brahma, Vijaya ^{[3
]}

Donaldson, Sylva ^{[2
]}

Zhang, Yu ^{[3
,4
]}

Surendra, Anu ^{[2
]}

Gong, Yunchen ^{[2
]}

Tullis, D. Elizabeth ^{[5
]}

Yau, Yvonne C. W. ^{[6
,7
]}

Waters, Valerie J. ^{[8
]}

Hwang, David M. ^{[3
,4
,7
]}

Guttman, David S. ^{[1
,2
]}

机构：

[1] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada

[2] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada

[3] Univ Toronto, Univ Hlth Network, Latner Thorac Surg Labs, Toronto, ON, Canada

[4] Univ Toronto, Dept Pathol, Univ Hlth Network, Toronto, ON, Canada

[5] St Michaels Hosp, Adult Cyst Fibrosis Clin, Toronto, ON M5B 1W8, Canada

[6] Hosp Sick Children, Dept Pediat Lab Med, Microbiol, Toronto, ON M5G 1X8, Canada

[7] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[8] Univ Toronto, Hosp Sick Children, Dept Pediat, Div Infect Dis, Toronto, ON M5G 1X8, Canada

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

加拿大健康研究院;

关键词：

BACTERIAL COMMUNITIES; AIRWAY MICROBIOTA; DIVERSITY; EXACERBATIONS; SURVIVAL; SEARCH; IMPACT; RANGE;

D O I：

10.1038/srep10241

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Understanding the significance of bacterial species that colonize and persist in cystic fibrosis (CF) airways requires a detailed examination of bacterial community structure across a broad range of age and disease stage. We used 16S ribosomal RNA sequencing to characterize the lung microbiota in 269 CF patients spanning a 60 year age range, including 76 pediatric samples from patients of age 4-17, and a broad cross-section of disease status to identify features of bacterial community structure and their relationship to disease stage and age. The CF lung microbiota shows significant inter-individual variability in community structure, composition and diversity. The core microbiota consists of five genera -Streptococcus, Prevotella, Rothia, Veillonella and Actinomyces. CF-associated pathogens such as Pseudomonas, Burkholderia, Stenotrophomonas and Achromobacter are less prevalent than core genera, but have a strong tendency to dominate the bacterial community when present. Community diversity and lung function are greatest in patients less than 10 years of age and lower in older age groups, plateauing at approximately age 25. Lower community diversity correlates with worse lung function in a multivariate regression model. Infection by Pseudomonas correlates with age-associated trends in community diversity and lung function.

引用

页数：12

共 35 条

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