Use of a real-time polymerase chain reaction thermocycler to study bacterial cell permeabilization by antimicrobial peptides

被引：15

作者：

Bourbon, Clarisse ^{[1
]}

Bry, Claire ^{[1
]}

Roggemans, Caroline ^{[1
]}

Soulard, Clelia ^{[1
]}

Thizon, Celine ^{[1
]}

Garbay, Bertrand ^{[1
]}

机构：

[1] Univ Bordeaux 2, Ecole Super Technol Biomol Bordeaux, F-33076 Bordeaux, France

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 381卷 / 02期

关键词：

D O I：

10.1016/j.ab.2008.07.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antimicrobial peptides are good leads to develop new antibiotics, but knowledge of their mode of action is a prerequisite. Destruction of the microbial membranes through a detergent-like mechanism is one of these modes of action. This is Usually studied by using a fluorescent nucleic acid Stain Such as SYTOX Green, which is impermeable to living cells. Using a simple protocol based oil the use of a standard real-time thermocycler, we confirmed that the actions of the antimicrobial peptides L-37 and magainin 2 oil bacterial cells are different. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：279 / 281

页数：3

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