Role and Regulation of the Flp/Tad Pilus in the Virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193

被引：35

作者：

Nykyri, Johanna ^{[1
]}

Mattinen, Laura ^{[1
]}

Niemi, Outi ^{[2
]}

Adhikari, Satish ^{[1
]}

Koiv, Viia ^{[3
]}

Somervuo, Panu ^{[4
]}

Fang, Xin ^{[1
]}

Auvinen, Petri ^{[4
]}

Mae, Andres ^{[3
]}

Palva, E. Tapio ^{[2
]}

Pirhonen, Minna ^{[1
]}

机构：

[1] Univ Helsinki, Dept Agr Sci, Helsinki, Finland

[2] Univ Helsinki, Dept Biosci, Helsinki, Finland

[3] Univ Tartu, Inst Mol & Cell Biol, EE-50090 Tartu, Estonia

[4] Univ Helsinki, Inst Biotechnol, Helsinki, Finland

来源：

PLOS ONE | 2013年 / 8卷 / 09期

基金：

芬兰科学院;

关键词：

WIDESPREAD COLONIZATION ISLAND; CAROTOVORA SUBSP ATROSEPTICA; PATHOGEN ERWINIA-CAROTOVORA; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; AGGREGATIBACTER-ACTINOMYCETEMCOMITANS; TAD LOCUS; NONSPECIFIC ADHERENCE; BIOFILM FORMATION; GENE-EXPRESSION; PROTEIN;

D O I：

10.1371/journal.pone.0073718

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study, we characterized a putative Flp/Tad pilus-encoding gene cluster, and we examined its regulation at the transcriptional level and its role in the virulence of potato pathogenic enterobacteria of the genus Pectobacterium. The Flp/Tad pilus-encoding gene clusters in Pectobacterium atrosepticum, Pectobacterium wasabiae and Pectobacterium aroidearum were compared to previously characterized flp/tad gene clusters, including that of the well-studied Flp/Tad pilus model organism Aggregatibacter actinomycetemcomitans, in which this pilus is a major virulence determinant. Comparative analyses revealed substantial protein sequence similarity and open reading frame synteny between the previously characterized flp/tad gene clusters and the cluster in Pectobacterium, suggesting that the predicted flp/tad gene cluster in Pectobacterium encodes a Flp/Tad pilus-like structure. We detected genes for a novel two-component system adjacent to the flp/tad gene cluster in Pectobacterium, and mutant analysis demonstrated that this system has a positive effect on the transcription of selected Flp/Tad pilus biogenesis genes, suggesting that this response regulator regulate the flp/tad gene cluster. Mutagenesis of either the predicted regulator gene or selected Flp/Tad pilus biogenesis genes had a significant impact on the maceration ability of the bacterial strains in potato tubers, indicating that the Flp/Tad pilus-encoding gene cluster represents a novel virulence determinant in Pectobacterium. Soft-rot enterobacteria in the genera Pectobacterium and Dickeya are of great agricultural importance, and an investigation of the virulence of these pathogens could facilitate improvements in agricultural practices, thus benefiting farmers, the potato industry and consumers.

引用

页数：11

共 43 条

[31] N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels [J].