Circulating cell-free DNA in serum as a biomarker of colorectal cancer

被引：39

作者：

da Silva Filho, Benisio Ferreira ^{[1
]}

Almeida Diniz Gurgel, Ana Pavla ^{[2
]}

de Freitas Lins Neto, Manoel Alvaro ^{[3
]}

de Azevedo, Dalmo Almeida ^{[1
]}

de Freitas, Antonio Carlos ^{[2
]}

Silva Neto, Jacinto da Costa ^{[4
]}

Ferreira Silva, Luiz Antonio ^{[1
]}

机构：

[1] Univ Fed Alagoas, Inst Biol Sci & Hlth, Dept Genet, Maceio, Brazil

[2] Univ Fed Pernambuco, Dept Genet, BR-50670901 Recife, PE, Brazil

[3] Univ Fed Alagoas, Fac Med, Maceio, Brazil

[4] Univ Fed Pernambuco, Dept Histol & Embriol, BR-50670901 Recife, PE, Brazil

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2013年 / 66卷 / 09期

关键词：

Colorectal Cancer; Molecular Pathology; PCR; MITOCHONDRIAL-DNA; DIAGNOSTIC-VALUE; TUMOR DNA; BENIGN; QUANTIFICATION; INTEGRITY;

D O I：

10.1136/jclinpath-2013-201521

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Aims The aim of this study was to report circulating cell-free DNA using ALU247 and ALU247/ALU115 biomarkers in serum of operated and non-operated patients with colorectal cancer (CRC). Methods To undertake this, 90 blood samples were collected, including 30 samples from healthy volunteers; 27 samples from CRC non-operated patients and 33 samples from CRC-operated patients. Circulating cell-free DNA was verified through quantitative real-time PCR (qPCR) using ALU115 and ALU247 primers. Results With regard to the ALU115-qPCR biomarker, the increased levels of circulating cell-free DNA in serum of non-operated patients were significant when compared with control (p<0.05). Moreover, levels of ALU247-qPCR biomarker were statistically significant between non-operated versus operated and non-operated versus control groups (p=0.000). With regard to the ALU247/115-qPCR biomarker, significant differences were observed between control versus non-operated patients (p=0.019), operated versus non-operated patients (p=0.005) and control versus operated patients (p=0.043). Conclusions Thus, the ALU247 and ALU247/ALU115-qPCR biomarkers may be important in detecting and monitoring CRC patients in both early and late stages.

引用

页码：775 / 778

页数：4

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