Pterostilbene Inhibits Human Multiple Myeloma Cells via ERK1/2 and JNK Pathway In Vitro and In Vivo

被引:42
作者
Xie, Bingqian [1 ]
Xu, Zhijian [2 ]
Hu, Liangning [1 ]
Chen, Gege [1 ]
Wei, Rong [1 ]
Yang, Guang [1 ]
Li, Bo [2 ]
Chang, Gaomei [1 ]
Sun, Xi [1 ]
Wu, Huiqun [1 ]
Zhang, Yong [2 ]
Dai, Bojie [3 ]
Tao, Yi [1 ]
Shi, Jumei [1 ]
Zhu, Weiliang [2 ]
机构
[1] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Dept Hematol, Shanghai 200072, Peoples R China
[2] Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
[3] Tongji Univ, Coll Life Sci & Technol, Shanghai 200092, Peoples R China
基金
中国国家自然科学基金;
关键词
multiple myeloma; pterostilbene; apoptosis; cell cycle; ERK1/2; JNK; NF-KAPPA-B; CANCER-CELLS; INDUCED APOPTOSIS; RESVERATROL; ARREST; EXPRESSION; RESISTANCE; PRODUCTS; GROWTH; DEATH;
D O I
10.3390/ijms17111927
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Multiple myeloma (MM) is the second most common malignancy in the hematologic system, which is characterized by accumulation of plasma cells in bone marrow. Pterostilbene (PTE) is a natural dimethylated analog of resveratrol, which has anti-oxidant, anti-inflammatory and anti-tumor properties. In the present study, we examined the anti-tumor effect of PTE on MM cell lines both in vitro and in vivo using the cell counting kit (CCK)-8, apoptosis assays, cell cycle analysis, reactive oxygen species (ROS) generation, JC-1 mitochondrial membrane potential assay, Western blotting and tumor xenograft models. The results demonstrated that PTE induces apoptosis in the H929 cell line and causes cell cycle arrest at G0/G1 phase by enhancing ROS generation and reducing mitochondrial membrane potential. The anti-tumor effect of PTE may be caused by the activation of the extracellular regulated protein kinases (ERK) 1/2 and c-Jun N-terminal kinase (JNK) signaling pathways. Additionally, mice treated with PTE by intraperitoneal injection demonstrated reduced tumor volume. Taken together, the results of this study indicate that the anti-tumor effect of PTE on MM cells may provide a new therapeutic option for MM patients.
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页数:13
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