Low-intensity pulsed ultrasound (LIPUS) stimulates mineralization of MC3T3-E1 cells through calcium and phosphate uptake

被引：24
作者：
Fioravante Tassinary, Joao Alberto [1 ,2 ]
Lunardelli, Adroaldo [2 ,3 ]
Basso, Bruno de Souza [2 ]
Dias, Henrique Bregolin [2 ]
Catarina, Anderson Velasque [2 ]
Stulp, Simone [1 ]
Haute, Gabriela Viegas [2 ]
Martha, Bianca Andrade [2 ]
da Silva Melo, Denizar Alberto [2 ]
Nunes, Fernanda Bordignon [2 ]
Fagundes Donadio, Marcio Vinicius [2 ]
de Oliveira, Jarbas Rodrigues [2 ]
机构：
[1] Univates, Lajeado, RS, Brazil
[2] Pontificia Univ Catolica Rio Grande do Sul, Lab Pesquisa Biofis Celular & Inflamacao, Ave Ipiranga 6681,Predio 12,Bloco C,Sala 221, BR-90619900 Porto Alegre, RS, Brazil
[3] Ctr Univ Ritter dos Reis UniRitter, Porto Alegre, RS, Brazil
来源：
ULTRASONICS | 2018年 / 84卷
关键词：
Low-intensity pulsed ultrasound; Osteoblast; Mineralization; Phosphate; Calcium; Hydroxyapatite; BONE-FORMATION; IN-VITRO; THERAPEUTIC ULTRASOUND; MATRIX MINERALIZATION; ALKALINE-PHOSPHATASE; DIFFERENTIATION; GROWTH; PROLIFERATION; OSTEOBLASTS; ACTIVATION;
D O I：
10.1016/j.ultras.2017.11.011
中图分类号：
O42 [声学];
学科分类号：
070206 ; 082403 ;
摘要：
The present study aimed to evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on preosteoblast mineralization using in vitro bioassays. Pre-osteoblastic MC3T3-E1 cells were exposed to LIPUS at 1 MHz frequency, 0.2 W/cm(2) intensity and 20% duty cycle for 30 min. The analyses were carried out up to 336 h (14 days) after exposure. The concentration of collagen, phosphate, alkaline phosphatase, calcium and transforming growth factor beta 1 (TGF-beta 1) in cell supernatant and the presence of calcium deposits in the cells were analyzed. Our results showed that LIPUS promotes mineralized nodules formation. Collagen, phosphate, and calcium levels were decreased in cell supernatant at 192 h after LIPUS exposure. However, alkaline phosphatase and TGF-beta 1 concentrations remained unchanged. Therapeutic pulsed ultrasound is capable of stimulating differentiation and mineralization of preosteoblastic MC3T3-E1 cells by calcium and phosphate uptake with consequent hydroxyapatite formation. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：290 / 295
页数：6
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