Characterization ofPseudomonas aeruginosaisolates from patients with endophthalmitis using conventional microbiologic techniques and whole genome sequencing

被引：6

作者：

Sengillo, Jesse D. ^{[1
]}

Duker, Jacob ^{[1
]}

Hernandez, Maribel ^{[1
]}

Maestre, Jorge ^{[1
]}

Reyes-Capo, Daniela ^{[1
]}

Patel, Annika ^{[1
]}

Watane, Arjun ^{[1
]}

Patel, Nimesh A. ^{[1
]}

Yannuzzi, Nicolas A. ^{[1
]}

Miller, Darlene ^{[1
]}

Flynn, Harry W., Jr. ^{[1
]}

机构：

[1] Univ Miami, Miller Sch Med, Bascom Palmer Eye Inst, Dept Ophthalmol, 900 NW 17th St, Miami, FL 33136 USA

来源：

JOURNAL OF OPHTHALMIC INFLAMMATION AND INFECTION | 2020年 / 10卷 / 01期

关键词：

Pseudomonas aeruginosa; Whole genome sequencing; Endophthalmitis; CULTURE-PROVEN ENDOPHTHALMITIS; PSEUDOMONAS-AERUGINOSA; ANTIBIOTIC SUSCEPTIBILITIES; INFECTIOUS ENDOPHTHALMITIS; CLINICAL-FEATURES; VISUAL OUTCOMES; VIRULENCE; SYSTEMS;

D O I：

10.1186/s12348-020-00216-0

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose To demonstrate antibiotic susceptibility and genomic virulence factor profiles ofPseudomonas aeruginosaisolates from patients with culture-confirmed endophthalmitis. Methods Clinical isolates from patients diagnosed with pseudomonas endophthalmitis were included. Laboratory antibiotic susceptibility testing and whole genome sequencing was performed on all isolates. Results In the current study, 8 patients had vitreous culture-confirmed endophthalmitis due toP. aeruginosa. All isolates were multi-drug resistant but sensitive to ceftazidime and each fluoroquinolone tested. Whole genome sequencing revealed a total of 179 unique genes. The most common type of virulence genes included those involved in adherence and the secretion system. Seven of 8 (88%) isolates were of the cytoinvasive phenotype (exoST) and no isolates containedexoU. Conclusions P. aeruginosaassociated endophthalmitis is often multi-drug resistant and demonstrates a variety of virulence factors with those involved in adherence and the secretion system being the most common.

引用

页数：6

共 33 条

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