Fluorescent aptamer-functionalized graphene oxide biosensor for label-free detection of mercury(II)

被引：195

作者：

Li, Ming ^{[1
,3
]}

Zhou, Xuejiao ^{[2
]}

Ding, Weiqiang ^{[3
]}

Guo, Shouwu ^{[2
]}

Wu, Nianqiang ^{[1
,3
]}

机构：

[1] W Virginia Univ, Dept Mech & Aerosp Engn, Morgantown, WV 26506 USA

[2] Shanghai Jiao Tong Univ, Natl Key Lab Micro Nano Fabricat Technol, Key Lab Thin Film & Microfabricat, Minist Educ,Res Inst Micro Nano Sci & Technol, Shanghai 200240, Peoples R China

[3] W Virginia Univ, WV Nano Initiat, Morgantown, WV 26506 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 41卷

基金：

美国国家科学基金会;

关键词：

Graphene oxide; Mercury; Sensor; Fluorescence; Detection; SURFACE-ENERGY TRANSFER; CDSE/ZNS QUANTUM DOTS; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; MERCURIC ION; HG2+; SENSOR; MONOLAYERS; PLATFORM; HG(II);

D O I：

10.1016/j.bios.2012.09.060

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Label-free fluorescent detection of Hg2+ has been realized via quenching of fluorescence of graphene oxide (GO). The water-soluble GO sheets, which are functionalized with single-stranded DNA aptamer, exhibit strong fluorescence emission at 600 nm under the excitation of 488 nm in the absence of He ions. When Hg2+ ions appear in the aqueous solution, Hg2+ ions are sandwiched between the hairpin-shaped double-stranded DNA due to the formation of the thymine-Hg2+-thymine complex, which holds the Hg2+ ions in proximity to the surface of GO sheets. As a result, the fluorescence emission of GO is quenched. The present GO-based sensor shows a limit of detection as low as 0.92 nM and excellent selectivity toward Hg2+ over a wide range of metal ions. The present work indicates that GO is a promising fluorescent probe for detection of metal ions and biomolecules. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：889 / 893

页数：5

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