Unexpected Electrophoretic Behavior of Complexes between Rod-like Virions and Bivalent Antibodies

被引:0
|
作者
Beloborodov, Stanislav S. [1 ,2 ]
Panferov, Vasily G. [3 ]
Safenkova, Irina V. [3 ]
Krylova, Svetlana M. [1 ,2 ]
Dzantiev, Boris B. [3 ]
Krylov, Sergey N. [1 ,2 ]
机构
[1] York Univ, Dept Chem, Toronto, ON M3J 1P3, Canada
[2] York Univ, Ctr Res Biomol Interact, Toronto, ON M3J 1P3, Canada
[3] Russian Acad Sci, AN Bach Inst Biochem, Biotechnol Res Ctr, Moscow 119071, Russia
基金
俄罗斯科学基金会; 加拿大自然科学与工程研究理事会;
关键词
AFFINITY CAPILLARY-ELECTROPHORESIS; INFLUENZA-VIRUS HEMAGGLUTININ; QUANTITATIVE-ANALYSIS; BINDING; RECEPTOR; NANOPARTICLES; RECOGNITION; MOBILITY; ACID;
D O I
10.1021/acs.analchem.6b03779
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here we report on the unexpected electrophoretic behavior of complexes between rod-like virus particles (virions) and bivalent antibodies. The multiple complexes formed by the virions and antibodies migrated with electrophoretic mobilities of much greater absolute values than those of the unbound virions or antibodies while typically complexes have mobilities intermediate to those of their components. We hypothesized that the mobilities of unusually high absolute values are caused by the cross-linking of virions by bivalent antibodies into aggregates with prominent side-to-side binding. Theoretically, the mobility of such aggregates should be proportional to the square root of the number of cross-linked virions. The formation of virion aggregates with prominent side-to-side binding was confirmed by atomic force microscopy. The dependence of the aggregate mobility on the number of cross-linked virions can be used to estimate this number.
引用
收藏
页码:11908 / 11912
页数:5
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