HnRNPL inhibits the osteogenic differentiation of PDLCs stimulated by SrCl2 through repressing Setd2

被引：22

作者：

Jia, Xiaoshi ^{[1
,2
]}

Miron, Richard J. ^{[3
]}

Yin, Chengcheng ^{[1
,2
]}

Xu, Hudi ^{[1
,2
]}

Luo, Tao ^{[4
]}

Wang, Jiwei ^{[4
]}

Jia, Rong ^{[1
,2
]}

Wu, Min ^{[5
]}

Zhang, Yufeng ^{[1
,2
]}

Li, Yuhong ^{[1
,2
]}

机构：

[1] Wuhan Univ, State Key Lab Breeding Base Basic Sci Stomatol Hu, Wuhan, Hubei, Peoples R China

[2] Wuhan Univ, Minist Educ, Key Lab Oral Biomed, Wuhan, Hubei, Peoples R China

[3] Nova Southeastern Univ, Coll Dent Med, Cell Therapy Inst, Dept Periodontol, Ft Lauderdale, FL 33314 USA

[4] Guangzhou Med Univ, Stomatol Hosp, Guangzhou Inst Oral Dis, Key Lab Oral Med, Guangzhou, Guangdong, Peoples R China

[5] Wuhan Univ, Coll Life Sci, Dept Biochem & Mol Biol, Hubei Key Lab Cell Homeostasis,Hubei Key Lab Dev, Wuhan, Hubei, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2019年 / 23卷 / 04期

基金：

中国国家自然科学基金;

关键词：

bioengineering; hnRNPL; osteoporosis; periodontal ligament stem cells; RNA splicing; BIOACTIVE GLASS SCAFFOLDS; MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; PERIODONTAL-LIGAMENT; STRONTIUM RANELATE; BONE; REGENERATION; PROLIFERATION; SR;

D O I：

10.1111/jcmm.14166

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Osteoporosis has been shown to intensify bone loss caused by periodontitis and both share common risk factors. One strategy utilized to manage the disease has been via the release of Sr ions by Strontium Ranelate having a direct effect on preventing osteoclast activation and promoting osteoblast differentiation. Previously we have developed and characterized porous Sr-mesoporous bioactive glass (Sr-MBG) scaffolds and demonstrated their ability to promote periodontal regeneration when compared to MBG alone. Our group further discovered a splicing factor, heterogeneous nuclear ribonucleoprotein L (hnRNPL), was drastically down-regulated in periodontal ligament stem cells (PDLCs) stimulated by Sr through the activation of AKT pathway. Furthermore, hnRNPL restrained the osteogenic differentiation of PDLCs through down-regulating H3K36me3-specific methyltransferase Setd2. The goal of the present study was to investigate the mechanism of periodontal regeneration stimulated by Sr It was first found that the epigenetic mechanism of splicing factor hnRNPL participated in the osteogenesis processing of PDLCs stimulated by SrCl2. Meanwhile, the different role of hnRNPL and SET domain containing 2 (Setd2) may provide some implication of the treatment of periodontitis patients simultaneously suffering from osteoporosis.

引用

页码：2667 / 2677

页数：11

共 39 条

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