Silencing of Target Chitinase Genes via Oral Delivery of dsRNA Caused Lethal Phenotypic Effects in Mythimna separata (Lepidoptera: Noctuidae)

被引：19

作者：

Cao, Budao ^{[1
]}

Bao, Wenhua ^{[1
]}

Wuriyanghan, Hada ^{[1
]}

机构：

[1] Inner Mongolia Univ, 235 West Coll Rd, Hohhot 010021, Inner Mongolia, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2017年 / 181卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Mythimna separata; Chitinase gene; RNA interference; dsRNA; Biocontrol method; RNA INTERFERENCE; INSECT PESTS; HELICOVERPA-ARMIGERA; COTTON BOLLWORM; DIET; EXPRESSION; DEFENSE; PLANTS;

D O I：

10.1007/s12010-016-2254-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mythimna separata walker (Lepidoptera: Noctuidae) is a polyphagous, migratory corn pest. Outbreak of M. separata has led to severe damage to corn production recently in China. RNAi (RNA interference) is a gene silencing technology applied both in model and non-model organisms, and it is especially useful for the latter in which the reverse genetic research tools are not available. RNAi approach was broadly investigated in many plant pathogens and was used for the generation of anti-pest transgenic plants. We are proposing to use this technology to silence M. separata endogenous genes, thereby, providing a biocontrol method for this insect. Feeding of dsRNAs for target Chitinase genes resulted in substantial decreases of their transcript levels in M. separata. Furthermore, silencing of target Chitinase genes led to phenotypic effects such as reduced body weight and increased mortality. Our study provided both reverse genetic research tool and potential control strategy for this insect species.

引用

页码：860 / 866

页数：7

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