FTIR and Proteome Analysis of Sunflower Seedling (Helianthus annuus L.) under Lead Stress

被引：4
|
作者：
Wang, Yushuang [1 ]
Yang, Renjie [2 ]
Ding, Lu [1 ]
Xie, Xiaodong [1 ]
Liu, Haixue [1 ,3 ]
机构：
[1] Tianjin Agr Univ, Coll Agron & Resources & Environm, Tianjin, Peoples R China
[2] Tianjin Agr Univ, Coll Engn & Technol, Tianjin, Peoples R China
[3] Tianjin Agr Univ, Lab Agr Anal, Tianjin, Peoples R China
来源：
2018 FIRST INTERNATIONAL CONFERENCE ON ENVIRONMENT PREVENTION AND POLLUTION CONTROL TECHNOLOGY (EPPCT 2018) | 2018年 / 199卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1088/1755-1315/199/3/032064
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The effects of lead stress on the dynamic changes of protein secondary structure and differential protein expression of sunflower seedlings in soil were studied using Fourier transform infrared spectroscopy (FTIR) and two-dimensional (2D) electrophoresis. Under lead stress treatment, the alpha-helices and beta-sheets of protein secondary structure for sunflower seedlings were changed. The content changes, 110% increased in alpha-helices of roots and 40% declined in beta-sheets of leaves, were observed, respectively. Furthermore, 11 differential protein spots were obtained by analyzing 2-DE gel images, while No. 9 were identified by MALDI-TOF/TOF MS, successfully.
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页数：6
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