Labeling Cytosolic Targets in Live Cells with Blinking Probes

被引：20

作者：

Xu, Jianmin ^{[1
]}

Chang, Jason ^{[1
]}

Yan, Qi ^{[7
]}

Dertinger, Thomas ^{[5
]}

Bruchez, Marcel P. ^{[6
,7
,8
]}

Weiss, Shimon ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Calif Nano Syst Inst, Los Angeles, CA 90095 USA

[5] SOFast GmbH, D-10999 Berlin, Germany

[6] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[7] Carnegie Mellon Univ, Dept Biol Sci, Pittsburgh, PA 15213 USA

[8] Sharp Edge Labs, Pittsburgh, PA 15203 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2013年 / 4卷 / 13期

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; COATED QUANTUM DOTS; FLUORESCENT PROTEIN; LOCALIZATION MICROSCOPY; LIVING CELLS; INTRACELLULAR DELIVERY; SURFACE-PROTEINS; CELLULAR TARGETS; FUSION PROTEINS; SUPERRESOLUTION;

D O I：

10.1021/jz400682m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.

引用

页码：2138 / 2146

页数：9

共 50 条

[31] In Situ Live-Cell Nucleus Fluorescence Labeling with Bioinspired Fluorescent Probes
Ding, Pan
Wang, Houyu
Song, Bin
Ji, Xiaoyuan
Su, Yuanyuan
He, Yao
ANALYTICAL CHEMISTRY, 2017, 89 (15) : 7861 - 7868
[32] NADH-Activated Dual-Channel Fluorescent Probes for Multicolor Labeling of Live Cells and Tumor Mimic Spheroids
Podder, Arup
Murali, Vishnu Priya
Deepika, Selvakumar
Dhamija, Avinash
Biswas, Shayeri
Maiti, Kaustabh K.
Bhuniya, Sankarprasad
ANALYTICAL CHEMISTRY, 2020, 92 (18) : 12356 - 12362
[33] Magnetic resonance imaging probes for labeling of chondrocyte cells
Liu, Gang
Xia, Chunchao
Wang, Zhiyong
Lv, Fei
Gao, Fabao
Gong, Qiyong
Song, Bin
Ai, Hua
Gu, Zhongwei
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2011, 22 (03) : 601 - 606
[34] Magnetic resonance imaging probes for labeling of chondrocyte cells
Gang Liu
Chunchao Xia
Zhiyong Wang
Fei Lv
Fabao Gao
Qiyong Gong
Bin Song
Hua Ai
Zhongwei Gu
Journal of Materials Science: Materials in Medicine, 2011, 22 : 601 - 606
[35] Selective labeling of proteins with chemical probes in living cells
Lin, Michael Z.
Wang, Lei
PHYSIOLOGY, 2008, 23 (03) : 131 - 141
[36] Screening Live Cells Using RNA Detection Probes
Lahm, Harald
Weldon, Don
AMERICAN LABORATORY, 2015, 47 (05) : 42 - 44
[37] Fluorescent probes for the imaging of lipid droplets in live cells
Tian, He, Jr.
Sedgwick, Adam C.
Han, Hai-Hao
Sen, Sajal
Chen, Guo-Rong
Zang, Yi
Sessler, Jonathan L.
James, Tony D.
Li, Jia
He, Xiao-Peng
COORDINATION CHEMISTRY REVIEWS, 2021, 427
[38] DETECTING AND TRACKING NONFLUORESCENT NANOPARTICLE PROBES IN LIVE CELLS
Wang, Gufeng
Fang, Ning
IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS: OPTICAL AND SPECTROSCOPIC TECHNIQUES, 2012, 504 : 83 - 108
[39] Selective protein labeling with nanometer precision in live cells
Wieneke R.
BIOspektrum, 2019, 25 (1) : 37 - 40
[40] Decoy probes to measure kinase activity in live cells
Parker, Laurie
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 251

← 1 2 3 4 5 →