Labeling Cytosolic Targets in Live Cells with Blinking Probes

被引：20

作者：

Xu, Jianmin ^{[1
]}

Chang, Jason ^{[1
]}

Yan, Qi ^{[7
]}

Dertinger, Thomas ^{[5
]}

Bruchez, Marcel P. ^{[6
,7
,8
]}

Weiss, Shimon ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Calif Nano Syst Inst, Los Angeles, CA 90095 USA

[5] SOFast GmbH, D-10999 Berlin, Germany

[6] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[7] Carnegie Mellon Univ, Dept Biol Sci, Pittsburgh, PA 15213 USA

[8] Sharp Edge Labs, Pittsburgh, PA 15203 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2013年 / 4卷 / 13期

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; COATED QUANTUM DOTS; FLUORESCENT PROTEIN; LOCALIZATION MICROSCOPY; LIVING CELLS; INTRACELLULAR DELIVERY; SURFACE-PROTEINS; CELLULAR TARGETS; FUSION PROTEINS; SUPERRESOLUTION;

D O I：

10.1021/jz400682m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.

引用

页码：2138 / 2146

页数：9

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