Development of a Coaxial 3D Printing Platform for Biofabrication of Implantable Islet-Containing Constructs

被引:76
作者
Liu, Xiao [1 ]
Carter, Sarah-Sophia D. [1 ,2 ]
Renes, Max Jurie [1 ,2 ]
Kim, Juewan [3 ]
Rojas-Canales, Darling Macarena [4 ,5 ]
Penko, Daniella [4 ,5 ]
Angus, Cameron [1 ]
Beirne, Stephen [1 ]
Drogemuller, Christopher John [4 ,5 ]
Yue, Zhilian [1 ]
Coates, Patrick T. [4 ,5 ]
Wallace, Gordon G. [1 ]
机构
[1] Univ Wollongong, ARC Ctr Excellence Electromat Sci, Intelligent Polymer Res Inst, Wollongong, NSW 2522, Australia
[2] Univ Med Ctr Utrecht, Dept Orthoped, NL-3508 GA Utrecht, Netherlands
[3] Univ Adelaide, Sch Biol Sci, Dept Mol & Cellular Biol, Adelaide, SA 5005, Australia
[4] Univ Adelaide, Dept Med, Adelaide, SA 5000, Australia
[5] Royal Adelaide Hosp, Cent Northern Adelaide Renal & Transplantat Serv, Adelaide, SA 5000, Australia
基金
澳大利亚研究理事会;
关键词
3D bioprinting; bioink development; cell encapsulation; pancreatic islet transplantation; ENDOTHELIAL PROGENITOR CELLS; TRANSPLANTATION; ENGRAFTMENT; HYDROGELS; MATRICES; SIZE;
D O I
10.1002/adhm.201801181
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Over the last two decades, pancreatic islet transplantations have become a promising treatment for Type I diabetes. However, although providing a consistent and sustained exogenous insulin supply, there are a number of limitations hindering the widespread application of this approach. These include the lack of sufficient vasculature and allogeneic immune attacks after transplantation, which both contribute to poor cell survival rates. Here, these issues are addressed using a biofabrication approach. An alginate/gelatin-based bioink formulation is optimized for islet and islet-related cell encapsulation and 3D printing. In addition, a custom-designed coaxial printer is developed for 3D printing of multicellular islet-containing constructs. In this work, the ability to fabricate 3D constructs with precise control over the distribution of multiple cell types is demonstrated. In addition, it is shown that the viability of pancreatic islets is well maintained after the 3D printing process. Taken together, these results represent the first step toward an improved vehicle for islet transplantation and a potential novel strategy to treat Type I diabetes.
引用
收藏
页数:12
相关论文
共 51 条
[41]   Medical progress: Islet transplantation as a treatment for diabetes - A work in progress [J].
Robertson, RP .
NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (07) :694-705
[42]  
Rui Y, 2012, BIOFABRICATION, V4
[43]   Encapsulated islets for diabetes therapy: History, current progress, and critical issues requiring solution [J].
Scharp, David W. ;
Marchetti, Piero .
ADVANCED DRUG DELIVERY REVIEWS, 2014, 67-68 :35-73
[44]  
Seetho IW, 2014, CLIN BIOCH METABOLIC, P305, DOI DOI 10.1016/B978-0-7020-5140-1.00016-X
[45]   Pancreatic islet macroencapsulation using microwell porous membranes [J].
Skrzypek, Katarzyna ;
Nibbelink, Milou Groot ;
van Lente, Jere ;
Buitinga, Mijke ;
Engelse, Marten A. ;
de Koning, Eelco J. P. ;
Karperien, Marcel ;
van Apeldoorn, Aart ;
Stamatialis, Dimitrios .
SCIENTIFIC REPORTS, 2017, 7
[46]  
Storrs R, 2001, ANN NY ACAD SCI, V944, P252
[47]   Current and Future Perspectives on Alginate Encapsulated Pancreatic Islet [J].
Strand, Berit L. ;
Coron, Abba E. ;
Skjak-Braek, Gudmund .
STEM CELLS TRANSLATIONAL MEDICINE, 2017, 6 (04) :1053-1058
[48]   Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease [J].
Stull, Natalie D. ;
Breite, Andrew ;
McCarthy, Robert ;
Tersey, Sarah A. ;
Mirmira, Raghavendra G. .
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2012, (67)
[49]   Hydrogel Templates for Rapid Manufacturing of Bioactive Fibers and 3D Constructs [J].
Tamayol, Ali ;
Najafabadi, Alireza Hassani ;
Aliakbarian, Bahar ;
Arab-Tehrany, Elmira ;
Akbari, Mohsen ;
Annabi, Nasim ;
Juncker, David ;
Khademhosseini, Ali .
ADVANCED HEALTHCARE MATERIALS, 2015, 4 (14) :2146-2153
[50]   Robust free-standing nanomembranes of organic/inorganic interpenetrating networks [J].
Vendamme, Richard ;
Onoue, Shin-Ya ;
Nakao, Aiko ;
Kunitake, Toyoki .
NATURE MATERIALS, 2006, 5 (06) :494-501