The interaction between Ku antigen and REF1 protein mediates negative gene regulation by extracellular calcium

被引：65
作者：
Chung, U
Igarashi, T
Nishishita, T
Iwanari, H
Iwamatsu, A
Suwa, A
Mimori, T
Hata, K
Ebisu, S
Ogata, E
Fujita, T
Okazaki, T
机构：
[1] UNIV TOKYO,SCH MED,DEPT INTERNAL MED 4,ENDOCRINE GENET & HYPERTENS UNIT,BUNKYO KU,TOKYO 112,JAPAN
[2] INST IMMUNOL,BUNKYO KU,TOKYO 112,JAPAN
[3] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,YOKOHAMA,KANAGAWA 236,JAPAN
[4] TOKYO METROPOLITAN OHTSUKA HOSP,TOSHIMA KU,TOKYO 170,JAPAN
[5] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN
[6] MITSUBISHI KAGAKU BIOCLIN LAB INC,ITABASHI KU,TOKYO 174,JAPAN
[7] HIGETA SHOYU CO LTD,RES LAB,CHOSHI,CHIBA 288,JAPAN
[8] CANC INST HOSP,TOSHIMA KU,TOKYO 170,JAPAN
[9] UNIV TOKYO,SCH MED,HLTH SERV CTR,BUNKYO KU,TOKYO 112,JAPAN
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 15期
关键词：
D O I：
10.1074/jbc.271.15.8593
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Through the specific binding of a negative calcium-responsive element to its binding protein in response to extracellular Ca (Ca-e(2+)), negative calcium-responsive element-bearing genes, such as the human parathyroid hormone gene, are negatively regulated by Ca-e(2+). The Ku antigen mediated negative gene regulation by Ca-e(2+) by interacting with a redox factor protein, REF1. Although sequence-nonspecific DNA binding activity of the Ku antigen has been well characterized, the mechanism of its sequence-specific DNA binding remained obscure. Here, we report that the specific binding of the Ku antigen to another protein, REF1, leads to DNA-protein complex formation with a novel sequence specificity and thereby regulates gene expression.
引用
收藏
页码：8593 / 8598
页数：6
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