Development of latex agglutination test for rapid detection of antibodies against Bovine herpesvirus 1 and Bovine herpesvirus 5 in cattle

被引：4

作者：

Fan, Qiang ^{[1
]}

Yao, Lan ^{[1
]}

Ding, Ming ^{[1
]}

Wang, Dan-Jing ^{[1
]}

Chen, Huan-Chun ^{[1
]}

Liu, Zheng-Fei ^{[1
]}

机构：

[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Vet Med, Wuhan 430070, Peoples R China

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2012年 / 24卷 / 06期

关键词：

Bovine herpesvirus 1; Bovine herpesvirus 5; cattle; latex agglutination test; BOVINE HERPESVIRUS TYPE-1; VIRUS; BHV-1; PROTECTION; DISEASE; ASSAY;

D O I：

10.1177/1040638712462376

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A latex agglutination test (LAT) based on sensitized polystyrene beads with inactivated Bovine herpesvirus 1 (BHV-1) particles was developed to detect serum antibodies against BHV-1 and Bovine herpesvirus 5 (BHV-5). Compared with a virus neutralization test using 252 serum samples, the sensitivity and specificity were 94.7% and 97.5%, respectively. Finally, 512 clinical serum samples from 3 major cattle-breeding provinces were monitored, and the overall positive rate was 34.57% (95% confidence interval: 30.45-38.69%). The results suggest that LAT has the potential to be a rapid method for the diagnosis of BHV-1 and BHV-5 infection in cattle herds.

引用

页码：1162 / 1165

页数：4