Site-specific cleavage of mutant ABL mRNA by DNAzyme is facilitated by peptide nucleic acid binding to RNA substrate

被引：4

作者：

Kim, Ji Eun ^{[1
]}

Yoon, Soojin ^{[1
]}

Mok, Hyejung ^{[1
]}

Jung, Woong ^{[2
]}

Kim, Dong-Eun ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

[2] Kyung Hee Univ Hosp Gangdong, Dept Emergency Med, Seoul 134727, South Korea

来源：

FEBS LETTERS | 2012年 / 586卷 / 21期

关键词：

DNAzyme; Peptide nucleic acid (PNA); RNA cleavage; Single nucleotide polymorphism (SNP); CHRONIC MYELOGENOUS LEUKEMIA; DNA; OLIGONUCLEOTIDES; REPLICATION; RECOGNITION; INHIBITION; DASATINIB; DUPLEX; LONG; PNA;

D O I：

10.1016/j.febslet.2012.09.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-cleaving DNAzymes were constructed to target the point mutation in the BCR-ABL transcript that causes imatinib resistance in leukemic cells. We examined the effect of 12mer peptide nucleic acids (PNAs) as facilitator oligonucleotides that bind to RNA substrate at the termini of the DNAzyme to improve DNAzyme-mediated cleavage of full-length RNA. When imatinib-resistant cells were transfected with the facilitator PNA and DNAzyme, DNAzyme activity was enhanced and the cells were sensitized to imatinib treatment. Thus, facilitator PNA may be used to enhance activity of antisense oligonucleotide targeting the full-length transcript. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3865 / 3869

页数：5

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