Pattern of Auxin and Cytokinin Responses for Shoot Meristem Induction Results from the Regulation of Cytokinin Biosynthesis by AUXIN RESPONSE FACTOR3

被引:207
作者
Cheng, Zhi Juan [1 ,3 ,4 ]
Wang, Liang [1 ]
Sun, Wei [1 ]
Zhang, Yan [1 ]
Zhou, Chao [1 ]
Su, Ying Hua [1 ]
Li, Wei [1 ]
Sun, Tian Tian [1 ]
Zhao, Xiang Yu [1 ]
Li, Xing Guo [1 ]
Cheng, Youfa [2 ]
Zhao, Yunde [2 ]
Xie, Qi [3 ,4 ]
Zhang, Xian Sheng [1 ]
机构
[1] Shandong Agr Univ, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
[2] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
[3] Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[4] Chinese Acad Sci, Natl Plant Gene Res Ctr, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
基金
中国国家自然科学基金;
关键词
YUCCA FLAVIN MONOOXYGENASES; GENE-EXPRESSION; CELL SPECIFICATION; FLORAL MERISTEM; WUSCHEL GENE; STEM-CELLS; ARABIDOPSIS; ROOT; REGENERATION; GROWTH;
D O I
10.1104/pp.112.203166
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
De novo organ regeneration is an excellent biological system for the study of fundamental questions regarding stem cell initiation, cell fate determination, and hormone signaling. Despite the general belief that auxin and cytokinin responses interact to regulate de novo organ regeneration, the molecular mechanisms underlying such a cross talk are little understood. Here, we show that spatiotemporal biosynthesis and polar transport resulted in local auxin distribution in Arabidopsis (Arabidopsis thaliana), which in turn determined the cytokinin response during de novo shoot regeneration. Genetic and pharmacological interference of auxin distribution disrupted the cytokinin response and ATP/ADP ISOPENTENYLTRANSFERASE5 (AtIPT5) expression, affecting stem cell initiation and meristem formation. Transcriptomic data suggested that AUXIN RESPONSE FACTOR3 (ARF3) mediated the auxin response during de novo organ regeneration. Indeed, mutations in ARF3 caused ectopic cytokinin biosynthesis via the misexpression of AtIPT5, and this disrupted organ regeneration. We further showed that ARF3 directly bound to the promoter of AtIPT5 and negatively regulated AtIPT5 expression. The results from this study thus revealed an auxin-cytokinin cross talk mechanism involving distinct intermediate signaling components required for de novo stem cell initiation and shed new light on the mechanisms of organogenesis in planta.
引用
收藏
页码:240 / 251
页数:12
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