Structure of the Bacillus subtilis peptide antibiotic subtilosin a determined by 1H-NMR and matrix assisted laser desorption/ionization time-of-flight mass spectrometry

被引:68
作者
Marx, R
Stein, T
Entian, KD
Glaser, SJ
机构
[1] Univ Frankfurt, Inst Mikrobiol, D-60439 Frankfurt, Germany
[2] Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany
[3] Tech Univ Munich, Inst Organ Chem & Biochem 2, D-85747 Garching, Germany
来源
JOURNAL OF PROTEIN CHEMISTRY | 2001年 / 20卷 / 06期
关键词
Bacillus subtilis; subtilosin; peptide antibiotic; MALDI-TOFMS; nuclear magnetic resonance;
D O I
10.1023/A:1012562631268
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Subtilosin A produced by Bacillus subtilis is a macrocyclic peptide antibiotic which comprises 35 amino acids. Its molecular mass (3399.7 Da), determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and chemical properties gave experimental support for unusual intramolecular linkages. The three-dimensional fold of native subtilosin in dimethylsulfoxide was determined from two-dimensional H-1-NMR spectra recorded at 600 MHz. Based on the backbone conformation, a structure for subtilosin A is presented which is characterized by three inter-residue bridges where two cysteines are linked with two phenylalanine residues, respectively, and a third cysteine is bound to a threonine residue.
引用
收藏
页码:501 / 506
页数:6
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