Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

被引：3
作者：
Nikolelis, Dimitrios P. [1 ]
Psaroudakis, Nikolas [3 ]
Michaloliakos, Antonis I. [1 ]
Nikoleli, Georgia-Paraskevi [2 ]
Scoullos, Michael [1 ]
机构：
[1] Univ Athens, Dept Chem, Environm Chem Lab, GR-15771 Athens, Greece
[2] Natl Tech Univ Athens, Lab Inorgan & Analyt Chem, Sch Chem Engn, Dept 1, GR-15780 Athens, Greece
[3] Univ Athens, Dept Chem, Lab Inorgan Chem, GR-15771 Athens, Greece
来源：
CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2013年 / 11卷 / 02期
关键词：
Congener; 77; Stabilized lipid films; Biosensor; Flow injection; Antibody regeneration; MICROWAVE-ASSISTED EXTRACTION; POLYCHLORINATED-BIPHENYLS; PCBS;
D O I：
10.2478/s11532-012-0157-4
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3',4,4' tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10(-8) to 10(-5) M, with a detection limit of ca. 10(-8) M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5.
引用
收藏
页码：320 / 323
页数：4
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