Chemical profiling of complex biochemical mixtures from various seaweeds

被引：35
作者：
Date, Yasuhiro [1 ,2 ]
Sakata, Kenji [2 ]
Kikuchi, Jun [1 ,2 ,3 ,4 ]
机构：
[1] RIKEN Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[2] Yokohama City Univ, Grad Sch Nanobiosci, Yokohama, Kanagawa 232, Japan
[3] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan
[4] RIKEN Res Cluster Innovat, Biomass Engn Program, Yokohama, Kanagawa, Japan
来源：
POLYMER JOURNAL | 2012年 / 44卷 / 08期
基金：
日本科学技术振兴机构;
关键词：
compositional profiling; multivariate statistical analysis; nuclear magnetic resonance (NMR); polysaccharide; seaweed biomass; METABOLIC DYNAMICS; NMR; PLANT;
D O I：
10.1038/pj.2012.105
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Development of an approach to describing and comparing complex biochemical mixtures presented by natural biomacromolecular sources is a promising new field in polymer science. The generation of a comprehensive strategy for evaluating and characterizing natural biomass mixtures presents a significant challenge. Here, we describe a chemical profiling methodology for seaweed biomass based on the comprehensive characterization of water-soluble, water-insoluble and solid-state components. An extraction method was developed for the nuclear magnetic resonance measurement of the water-insoluble seaweed components by modifying and optimizing physical pretreatments and extraction processes. Evaluating the compositional variations in various seaweeds from biomass profiles by principal component analysis score plots showed that the species clustered according to their taxonomic group differences. In addition, this profiling strategy revealed that it was possible to annotate the primary components that are characteristic of each taxonomic group, such as mannitol and laminaran in brown algae, D-galactose and agar in red algae, and xylopyranose and glucopyranose in green algae. Therefore, this profiling strategy was capable of discerning and evaluating individual characteristics among these seaweeds. Polymer Journal (2012) 44, 888-894; doi:10.1038/pj.2012.105; published online 13 June 2012
引用
收藏
页码：888 / 894
页数：7
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