Highly specific and sensitive immunoassay for the measurement of prostaglandin E2 in biological fluids

被引：4

作者：

Gao, Yuan ^{[1
,2
,3
]}

Hou, Rui ^{[1
,2
]}

Liu, Fen ^{[1
,2
]}

Cai, Runlan ^{[1
,2
]}

Fang, Lei ^{[1
,2
]}

Peng, Cheng ^{[3
]}

Qi, Yun ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China

[2] Peking Union Med Coll, Beijing 100193, Peoples R China

[3] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Peoples R China

来源：

BIOANALYSIS | 2015年 / 7卷 / 19期

基金：

北京市自然科学基金;

关键词：

BOVINE SERUM-ALBUMIN; MONOCLONAL-ANTIBODY; PROTEIN ANTIGENS; E SYNTHASE; INHIBITION; ENZYME; RADIOIMMUNOASSAY; CATIONIZATION; HAPTEN; CONJUGATE;

D O I：

10.4155/bio.15.130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Lack of specificity of anti-PGE(2) antibodies is a long-standing problem. Given quite a few analogs and low PGE(2) content in biological fluids, it is quite important to simultaneously meet the demands of high specificity and sensitivity. Results: Highly specific anti-PGE(2) antibodies were obtained by combined use of cationic carrier protein and Mannich reaction. The cross-reactivity values of the resultant polyclonal and monoclonal antibodies against eight analogs were <14 and <5%, respectively. Furthermore, we established a highly sensitive ELISA, which could be applied to direct analysis of PGE(2) at the pg/ml level (LOQ = 15.6 pg/ml). Conclusion: We provide an appropriate strategy to develop a highly-specific and sensitive immunoassay for measuring low PGE(2) content in biological samples.

引用

页码：2597 / 2607

页数：11

共 36 条

[31] QUANTITATION OF EPSILON-AMINO GROUP USING AMINO-ACIDS AS REFERENCE-STANDARDS BY TRINITROBENZENE SULFONIC-ACID - A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF HAPTEN TO CARRIER PROTEIN RATIO [J].

SASHIDHAR, RB ;

CAPOOR, AK ;

RAMANA, D .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 167 (1-2) :121-127

[32]

TANAKA T, 1985, BIOCHIM BIOPHYS ACTA, V836, P125

[33] Coordinate up- and down-regulation of glutathione-dependent prostaglandin E synthase and cyclooxygenase-2 in A549 cells -: Inhibition by NS-398 and leukotriene C4 [J].

Thorén, S ;

Jakobsson, PJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (21) :6428-6434

[34]

Watanabe S, 1997, BIOL PHARM BULL, V20, P101

[35] Preparation for aflatoxin B1-cationized bovine serum albumin based on Mannich-type reaction [J].

Zhou, Youxiang ;

Wu, Jiajia ;

Yu, Wei ;

Xu, Ying ;

Wang, Ping ;

Xie, Bijun ;

Chen, Fusheng .

JOURNAL OF IMMUNOLOGICAL METHODS, 2007, 328 (1-2) :79-88

[36] RADIOIMMUNOASSAY OF A-PROSTAGLANDINS [J].

ZUSMAN, RM ;

SPEROFF, L ;

CALDWELL, BV ;

BEHRMAN, HR .

PROSTAGLANDINS, 1972, 2 (01) :41-&

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