Biosensors Based on Enzyme Inhibition

被引：26

作者：

Arduini, Fabiana ^{[1
,2
]}

Amine, Aziz ^{[3
]}

机构：

[1] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

[2] Consorzio Interuniv Biostrutture & Biosistemi INB, Rome, Italy

[3] Fac Sci & Tech, Mohammadia, Morocco

来源：

BIOSENSORS BASED ON APTAMERS AND ENZYMES | 2014年 / 140卷

关键词：

Biosensor; Enzyme; Inhibition; SCREEN-PRINTED ELECTRODES; NERVE AGENTS; AMPEROMETRIC BIOSENSOR; ORGANOPHOSPHATE PESTICIDES; ELECTROCHEMICAL DETECTION; GLUCOSE-OXIDASE; CHOLINESTERASE BIOSENSOR; TYROSINASE ENZYME; CARBON NANOTUBES; ACETYLCHOLINESTERASE;

D O I：

10.1007/10_2013_224

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present chapter describes the use of biosensors based on enzyme inhibition as analytical tools. The parameters that affect biosensor sensitivity, such as the amount of immobilized enzyme, incubation time, and immobilization type, were critically evaluated, highlighting how the knowledge of enzymatic kinetics can help researchers optimize the biosensor in an easy and fast manner. The applications of these biosensors demonstrating their wide application have been reported. The objective of this survey is to give a critical description of biosensors based on enzyme inhibition, of their assembly, and their application in the environmental, food, and pharmaceutical fields.

引用

页码：299 / 326

页数：28

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