Isolation and Amino Acid Sequencing by MALDI-TOF-MS/MS of a Novel Antimicrobial Anionic Peptide from the Skin Secretion of Osteocephalus taurinus (Anura, Hylidae)

被引：8

作者：

Costa, Tulio O. G. ^{[1
,2
]}

Almeida, Richardson A. ^{[1
]}

Melo, Jorge T. ^{[3
]}

Koolen, Hector H. F. ^{[4
]}

da Silva, Felipe M. A. ^{[1
]}

Leite, Jose Roberto S. A. ^{[5
]}

Prates, Maura V. ^{[3
]}

Bloch, Carlos, Jr. ^{[3
]}

Pinto, Angelo C. ^{[2
]}

机构：

[1] Univ Fed Amazonas, Dept Quim, BR-69077000 Manaus, Amazonas, Brazil

[2] Univ Fed Rio de Janeiro, Inst Quim CT, BR-21945970 Rio De Janeiro, RJ, Brazil

[3] EMBRAPA Recursos Genet & Biotecnol, BR-70770900 Brasilia, DF, Brazil

[4] Univ Estadual Campinas, Intituto Quim, BR-13084971 Campinas, SP, Brazil

[5] Univ Fed Piaui, Biotec, BR-64202020 Parnaiba, PI, Brazil

来源：

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY | 2012年 / 23卷 / 12期

关键词：

anionic peptide; antimicrobial peptide; Osteocephalus taurinus; skin secretion; ANTIBACTERIAL;

D O I：

10.1590/S0103-50532012001200002

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.

引用

页码：2133 / 2136

页数：4

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