Effect of ginseng extract on the TGF-β1 signaling pathway in CCl4-induced liver fibrosis in rats

被引：58
作者：
Hafez, Mohamed M. [1 ]
Hamed, Sherifa S. [2 ,3 ]
El-Khadragy, Manal F. [2 ,4 ]
Hassan, Zeinab K. [5 ]
Al Rejaie, Salim S. [1 ]
Sayed-Ahmed, Mohamed M. [1 ]
Al-Harbi, Naif O. [1 ]
Al-Hosaini, Khalid A. [1 ]
Al-Harbi, Mohamed M. [1 ]
Alhoshani, Ali R. [1 ]
Al-Shabanah, Othman A. [1 ]
Alsharari, Shakir Dekhal [1 ]
机构：
[1] King Saud Univ, Dept Pharmacol & Toxicol, Coll Pharm, POB 2457, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Dept Zool, Coll Sci, Riyadh, Saudi Arabia
[3] Univ Alexandria, Dept Zool, Coll Sci, Alexandria, Egypt
[4] Helwan Univ, Coll Sci, Dept Zool & Entomol, Cairo, Egypt
[5] Cairo Univ, Natl Canc Inst, Virol & Immunol Unit, Dept Canc Biol, Cairo, Egypt
来源：
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2017年 / 17卷
关键词：
Ginseng extract; Carbon tetrachloride; Gene expression; Real time PCR; INDUCED LIPID-PEROXIDATION; PANAX-GINSENG; CARBON-TETRACHLORIDE; CELL ACTIVATION; MATRIX METALLOPROTEINASES; ISCHEMIA-REPERFUSION; COMPOUND-K; TGF-BETA; HEPATOTOXICITY; EXPRESSION;
D O I：
10.1186/s12906-016-1507-0
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Background: Liver diseases are major global health problems. Ginseng extract has antioxidant, immune-modulatory and anti-inflammatory activities. This study investigated the effect of ginseng extract on carbon tetrachloride (CCl4)induced liver fibrosis in rats. Methods: Male Wistar rats were divided into four groups: control group, ginseng group, CCl4 group and CCl4+ ginseng group. Liver injury was induced by the intraperitoneal (I.P) injection of 3 ml/kg CCl4 (30% in olive oil) weekly for 8 weeks. The control group was I.P injected with olive oil. The expression of genes encoding transforming growth factor beta (TGF-beta), type I TGF-beta receptor (T beta R-1), type II TGF-beta receptor (T beta R-II), mothers against decapentaplegic homolog 2 (Smad2), Smad3, Smad4, matrix metalloproteinase 2 (MMP2), MMP9, tissue inhibitor matrix metalloproteinase-1 (TIMP-1), Collagen 1a2 (Col1a2), Collagen 3a1 (Col3a1), interleukin-8 (IL-8) and interleukin -10 (IL-10) were measured by real-time PCR. Results: Treatment with ginseng extract decreased hepatic fat deposition and lowered hepatic reticular fiber accumulation compared with the CCl4 group. The CC(l)4 group showed a significant increase in hepatotoxicity biomarkers and up-regulation of the expression of genes encoding TGF-beta, T beta R-I, T beta R-II, MMP2, MMP9, Smad-2,3, -4, and IL-8 compared with the control group. However, CCl4 administration resulted in the significant down-regulation of IL-10 mRNA expression compared with the control group. Interestingly, ginseng extract supplementation completely reversed the biochemical markers of hepatotoxicity and the gene expression alterations induced by CCl4. Conclusion: ginseng extract had an anti-fibrosis effect via the regulation of the TGF-beta 1/Smad signaling pathway in the CCl4-induced liver fibrosis model. The major target was the inhibition of the expression of TGF-beta 1, Smad2, and Smad3.
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页数：11
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