Type III apparatus of Pseudomonas aeruginosa as a tool to diagnose pulmonary infection in cystic fibrosis patients

被引：3

作者：

Cruz, Aline C. ^{[1
]}

Neves, Bianca C. ^{[2
]}

Higa, Laurinda Y. S. ^{[3
]}

Folescu, Tania ^{[3
]}

Marques, Elizabeth A. ^{[1
]}

Milagres, Lucimar G. ^{[1
]}

机构：

[1] Univ Estado Rio de Janeiro, Dept Microbiol Imunol & Parasitol, BR-20550170 Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Inst Chem, Dept Biochem, Rio De Janeiro, RJ, Brazil

[3] Fiocruz MS, Fernandes Figueira Inst, Dept Pulmonol, BR-21045900 Rio De Janeiro, RJ, Brazil

来源：

APMIS | 2012年 / 120卷 / 08期

关键词：

Antibody response; P; aeruginosa; pulmonary infection; diagnosis; cystic fibrosis; V-ANTIGEN; CHILDREN;

D O I：

10.1111/j.1600-0463.2012.02888.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Pseudomonas aeruginosa is associated with increased mortality in cystic fibrosis (CF) patients, and expresses type III secretion system proteins (TTSP), which is a common mechanism used by gram-negative pathogens for delivery of anti-host factors. Our aim was to investigate whether or not these antigens (TTSP) would be recognized by CF sera, by Western blot reaction. We have showed herein that all patients (n = 11) not chronically infected by P. aeruginosa had their first serum positive for TTSP (ExoS, ExoT, PopB, and/or PopD). All chronic patients had a strong positive serology to TTSP, although relatively weak reactions to TTSP were observed for some individuals in the negative control group. Therefore, TTSP that were early produced in P. aeruginosa infected CF patients, induced a detectable antibody response in those patients and were easily detected by Western-blot reaction.

引用

页码：622 / 627

页数：6

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