IL-33 promotes eosinophilia in vivo and antagonizes IL-5-dependent eosinophil hematopoiesis ex vivo

被引:33
作者
Dyer, Kimberly D. [1 ]
Percopo, Caroline M. [1 ]
Rosenberg, Helene F. [1 ]
机构
[1] NIAID, Lab Allerg Dis, Inflammat Immunobiol Sect, NIH, Bethesda, MD 20892 USA
关键词
Eosinophil; IL-5; IL-33; GM-CSF; MOUSE BONE-MARROW; AIRWAY INFLAMMATION; CYTOKINE IL-33; STEM-CELLS; RECEPTOR; ST2; MACROPHAGES; INTERLEUKIN-33; EXPANSION; LIGAND;
D O I
10.1016/j.imlet.2012.12.002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
IL-33 is an IL-1 family cytokine that elicits IL-5-dependent eosinophilia in vivo. We show here that IL-33 promotes minimal eosinophil hematopoiesis via direct interactions with mouse bone marrow progenitors ex vivo and that it antagonizes eosinophil hematopoiesis promoted by IL-5 on SCF and Flt3L primed bone marrow progenitor cells in culture. SCF and Flt3L primed progenitors respond to IL-33 by acquiring an adherent, macrophage-like phenotype, and by releasing macrophage-associated cytokines into the culture medium. IL-33-mediated antagonism of IL-5 was reproduced in part by the addition of GM-CSF and was inhibited by the actions of neutralizing anti-GM-CSF antibody. These findings suggest that the direct actions of IL-33 on bone marrow progenitors primed with SCF and Flt3L are antagonistic to the actions of IL-5 and are mediated in part by GM-CSF. Published by Elsevier B.V.
引用
收藏
页码:41 / 47
页数:7
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