Modulating the behaviors of C3A cells via surface charges of polyelectrolyte multilayers

被引：5

作者：

Huang, Xiaobo ^{[1
]}

Lin, Naiming ^{[1
]}

Hang, Ruiqiang ^{[1
]}

Wang, Xiaoguang ^{[1
]}

Zhang, Xiangyu ^{[1
]}

Tang, Bin ^{[1
]}

机构：

[1] Taiyuan Univ Technol, Inst Surface Engn, Taiyuan 030024, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 02期

关键词：

PEMs; Multilayer film; Charge; Liver tissue engineering; ADSORPTION; HYDROGELS; DENSITY; LIVER;

D O I：

10.1016/j.carbpol.2012.10.063

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The purpose of this study was to evaluate in vitro how the modulating surface charges of materials influenced the behaviors of hepatocytes. Cells of a human hepatocyte line, C3A, which have been used in a clinically tested bioartificial liver, were conducted as cell models. Polyelectrolyte multilayers (PEMs) of poly-L-lysine and alginate biopolymers were fabricated and then the zeta potential was assessed. Protein adsorption study showed that fibrinogen deposition could be modulated via tuning the terminal layer and the surface charges of PEMs. Furthermore, through observing the cellular morphology, viability, functional protein analysis and gene expression, we found that the behavior of C3A cells could be modulated via tuning of surface charges on PEMs, which was different from that via grafting functional groups on PEMs. It suggested that the PEMs with different charges could be used in vitro to manipulate cell behaviors to improve upon the design of tissue engineering. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1064 / 1070

页数：7

共 31 条

[1] Controlling mammalian cell interactions on patterned polyelectrolyte multilayer surfaces [J].

Berg, MC ;

Yang, SY ;

Hammond, PT ;

Rubner, MF .

LANGMUIR, 2004, 20 (04) :1362-1368

[2] Modulation of hepatocyte phenotype in vitro via chemomechanical tuning of polyelectrolyte multilayers [J].

Chen, Alice A. ;

Khetani, Salman R. ;

Lee, Sunyoung ;

Bhatia, Sangeeta N. ;

Van Vliet, Krystyn J. .

BIOMATERIALS, 2009, 30 (06) :1113-1120

[3] Dynamic cell behavior on synthetic hydrogels with different charge densities [J].

Chen, Yong Mei ;

Ogawa, Rie ;

Kakugo, Akira ;

Osada, Yoshihito ;

Gong, Jian Ping .

SOFT MATTER, 2009, 5 (09) :1804-1811

[4] Enhancing the drug metabolism activities of C3A - A human hepatocyte cell line - By tissue engineering within alginate scaffolds [J].

Elkayam, Tsiona ;

Amitay-Shaprut, Sigalit ;

Dvir-Ginzberg, Mona ;

Harel, Tamar ;

Cohen, Smadar .

TISSUE ENGINEERING, 2006, 12 (05) :1357-1368

[5]

Glicklis R, 2000, BIOTECHNOL BIOENG, V67, P344, DOI 10.1002/(SICI)1097-0290(20000205)67:3<344::AID-BIT11>3.0.CO

[6]

2-2

[7] Capturing complex 3D tissue physiology in vitro [J].

Griffith, LG ;

Swartz, MA .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2006, 7 (03) :211-224

[8] Bioactive coatings based on a polyelectrolyte multilayer architecture functionalized by embedded proteins [J].

Jessel, N ;

Atalar, F ;

Lavalle, P ;

Mutterer, J ;

Decher, G ;

Schaaf, P ;

Voegel, JC ;

Ogier, J .

ADVANCED MATERIALS, 2003, 15 (09) :692-695

[9] Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function [J].

Khalil, M ;

Shariat-Panahi, A ;

Tootle, R ;

Ryder, T ;

McCloskey, P ;

Roberts, E ;

Hodgson, H ;

Selden, C .

JOURNAL OF HEPATOLOGY, 2001, 34 (01) :68-77

[10] Design of artificial extracellular matrices for tissue engineering [J].

Kim, Byung-Soo ;

Park, In-Kyu ;

Hoshiba, Takashi ;

Jiang, Hu-Lin ;

Choi, Yun-Jaie ;

Akaike, Toshihiro ;

Cho, Chong-Su .

PROGRESS IN POLYMER SCIENCE, 2011, 36 (02) :238-268

← 1 2 3 4 →