Recombination Form and Epidemiology of HIV-1 Unique Recombinant Strains Identified in Yunnan, China

被引：34

作者：

Li, Lin ^{[1
]}

Chen, Lili ^{[1
,3
]}

Yang, Shaomin ^{[2
]}

Li, Tianyi ^{[1
]}

Li, Jianjian ^{[2
]}

Liu, Yongjian ^{[1
]}

Jia, Lei ^{[1
]}

Yang, Bihui ^{[2
]}

Bao, Zuoyi ^{[1
]}

Li, Hanping ^{[1
]}

Wang, Xiaolin ^{[1
]}

Zhuang, Daomin ^{[1
]}

Liu, Siyang ^{[1
]}

Li, Jingyun ^{[1
]}

机构：

[1] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Dept AIDS Res, Beijing, Peoples R China

[2] Yunnan Prov Hosp Infect Dis, AIDS Care Ctr YNACC, Kunming, Yunnan, Peoples R China

[3] Urumqi Gen Hosp, Lanzhou Mil Area Command, Urumqi, Xinjiang, Peoples R China

来源：

PLOS ONE | 2012年 / 7卷 / 10期

基金：

中国国家自然科学基金;

关键词：

REVERSE TRANSCRIPTION; INTERSUBTYPE RECOMBINANTS; RETROVIRAL RECOMBINATION; TYPE-1; GENOME; DRUG-USE; INFECTION; PROVINCE;

D O I：

10.1371/journal.pone.0046777

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several studies identified HIV-1 recombination in some distinct areas in Yunnan, China. However, no comprehensive studies had been fulfilled in the whole province up to now. To illustrate the epidemiology and recombination form of Unique Recombinant Forms (URFs) circulating in Yunnan, 788 HIV-1 positive individuals residing in 15 prefectures of Yunnan were randomly enrolled into the study. Full-length gag and pol genes were amplified and sequenced. Maximum likelihood tree was constructed for phylogenetic analysis. Recombinant breakpoints and genomic schematics were identified with online software jpHMM. 63 (10.2%) unique recombinant strains were identified from 617 strains with subtypes. The URFs distributed significantly differently among prefectures (Pearson chi-square test, P<0.05). IDUs contained more URFs than sexual transmitted population (Pearson chi-square test, P<0.05). Two main recombinant forms were identified by considering the presence of CRF01_AE segments in full length gag-pol genes, which were B'/C and B'/C/CRF01-AE recombinants. Three clusters were identified in the ML tree which contained more than three sequences and supported by high bootstrap values. One CRF was identified. Many of URFs contained identical breakpoints. The results will contribute to our understanding on HIV recombination and provide clues to the identification of potential CRFs in China.

引用

页数：7

共 26 条

[1]

Chen LL, 2011, AIDS RES HUM RETROV, V27, P693, DOI [10.1089/AID.2010.0264, 10.1089/aid.2010.0264]

[2] RETROVIRAL RECOMBINATION DURING REVERSE TRANSCRIPTION [J].

GOODRICH, DW ;

DUESBERG, PH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) :2052-2056

[3] Cloning and characterization of a virtually full-length HIV type 1 genome from a subtype B′-Thai strain representing the most prevalent B-clade isolate in China [J].

Graf, M ;

Shao, Y ;

Zhao, Q ;

Seidl, T ;

Köstler, J ;

Wolf, H ;

Wagner, R .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1998, 14 (03) :285-288

[4] A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood [J].

Guindon, S ;

Gascuel, O .

SYSTEMATIC BIOLOGY, 2003, 52 (05) :696-704

[5] Global trends in molecular epidemiology of HIV-1 during 2000-2007 [J].

Hemelaar, Joris ;

Gouws, Eleanor ;

Ghys, Peter D. ;

Osmanov, Saladin .

AIDS, 2011, 25 (05) :679-689

[6] RETROVIRAL RECOMBINATION AND REVERSE TRANSCRIPTION [J].

HU, WS ;

TEMIN, HM .

SCIENCE, 1990, 250 (4985) :1227-1233

[7] High genetic diversity of HIV-1 was found in men who have sex with men in Shijiazhuang, China [J].

Li, Lin ;

Lu, Xinli ;

Li, Hanping ;

Chen, Lili ;

Wang, Zheng ;

Liu, Yongjian ;

Bao, Zuoyi ;

Li, Tianyi ;

Tian, Chong ;

Liu, Hongxin ;

Zhuang, Daomin ;

Liu, Siyang ;

Li, Jingyun .

INFECTION GENETICS AND EVOLUTION, 2011, 11 (06) :1487-1492

[8] Genetic Characterization of 13 Subtype CRF01_AE Near Full-Length Genomes in Guangxi, China [J].

Li, Lin ;

Liang, Shujia ;

Chen, Lili ;

Liu, Wei ;

Li, Hanping ;

Liu, Yongjian ;

Bao, Zuoyi ;

Wang, Zheng ;

Zhuang, Daomin ;

Liu, Siyang ;

Li, Jingyun .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2010, 26 (06) :699-704

[9] Identification and Characterization of Two New HIV Type 1 Unique (B/C) Recombinant Forms in China [J].

Liu, Yongjian ;

Li, Lin ;

Yang, Shaomin ;

Bao, Zuoyi ;

Li, Hanping ;

Wang, Zheng ;

Zhuang, Daomin ;

Liu, Siyang ;

Chen, Lili ;

Fan, Yishan ;

Zhong, Min ;

Gao, Li ;

Wang, Xiaolin ;

Li, Jingyun .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2011, 27 (04) :445-451

[10] HIV-1 SUBTYPE-C IN CHINA [J].

LUO, CC ;

TIAN, CQ ;

HU, DJ ;

KAI, M ;

DONDERO, TJ ;

ZHENG, XW ;

LIU, XL ;

CHEN, J ;

LI, DQ ;

QU, SQ ;

ZHANG, GY ;

GEORGE, JR ;

SCHOCHETMAN, G ;

KALISH, ML .

LANCET, 1995, 345 (8956) :1051-1052

← 1 2 3 →