Tailoring an alien ferredoxin to support native-like P450 monooxygenase activity

被引：30

作者：

Bell, Stephen G. ^{[1
,2
]}

McMillan, James H. C. ^{[2
]}

Yorke, Jake A. ^{[2
]}

Kavanagh, Emma ^{[2
]}

Johnson, Eachan O. D. ^{[2
]}

Wong, Luet-Lok ^{[2
]}

机构：

[1] Univ Adelaide, Sch Chem & Phys, Adelaide, SA 5005, Australia

[2] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 95期

基金：

加拿大自然科学与工程研究理事会; 英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

ELECTRON-TRANSFER PROTEIN; CRYSTAL-STRUCTURE; CYP199A2; SYSTEM; ADRENODOXIN; CYTOCHROME-P450; CLUSTER; IDENTIFICATION;

D O I：

10.1039/c2cc35968e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A ferredoxin associated with biological Fe-S cluster assembly has been remodelled to transfer electrons to a P450 enzyme and support substrate oxidation at 80% of the physiological ferredoxin activity, opening up the possibility of tailoring ferredoxins to reconstitute the activity of P450 enzymes for which the electron transfer partner proteins are not known.

引用

页码：11692 / 11694

页数：3

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