Structural and mechanical characterization of crosslinked and sterilised nanocellulose-based hydrogels for cartilage tissue engineering

被引：64

作者：

Al-Sabanh, Ayesha ^{[1
]}

Burnell, Stephanie E. A. ^{[1
]}

Simoes, Irina N. ^{[1
]}

Jessop, Zita ^{[1
,2
]}

Badiei, Nafiseh ^{[3
]}

Blain, Emma ^{[4
]}

Whitaker, Iain S. ^{[1
,2
]}

机构：

[1] Swansea Univ, Inst Life Sci, Reconstruct Surg & Regenerat Med Grp ReconRegen, Med Sch, Swansea SA2 8PP, W Glam, Wales

[2] Morriston Hosp, Welsh Ctr Burns & Plast Surg, Swansea SA6 6NL, W Glam, Wales

[3] Swansea Univ, NanoHlth Ctr, Inst Life Sci, Med Sch, Swansea SA2 8QA, W Glam, Wales

[4] Cardiff Univ, Arthrit Res UK Biomech & Bioengn Ctr, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales

来源：

CARBOHYDRATE POLYMERS | 2019年 / 212卷

基金：

英国医学研究理事会;

关键词：

Nanocellulose; Crosslinking; Sterilisation; Hydrogels; Cartilage tissue engineering; PORE-SIZE; LIGHT-SCATTERING; CHONDROCYTES; ALGINATE; CELLULOSE; SCAFFOLD; CELLS; DIFFERENTIATION; BEHAVIOR; SURFACE;

D O I：

10.1016/j.carbpol.2019.02.057

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Nanocellulose is a natural biopolymer derived from cellulose. Combined with sodium alginate, it is used to 3D print hydrogels for articular and nasal cartilage engineering and shows good integration, promising cartilage regeneration and mechanical stability over 60 days of implantation in mice. Yet, little is known about their structural and mechanical properties, particularly the impact of crosslinking and sterilisation methods. This study investigates the impact of different calcium chloride crosslinker concentrations and sterilization methods on the structural and mechanical properties of nanocellulose-based hydrogels containing plant-derived cellulose nanofibrils, cellulose nanocrystals or a blend of the two. Crosslinking significantly alters the overall network distribution, surface morphology, pore size and porosity of the hydrogels. Sterilisation has a striking effect on pore size and affects swelling depending on the sterilisation method. The effect of crosslinker and sterilisation on the overall properties of the hydrogels was reliant on the form of nanocellulose that comprised them.

引用

页码：242 / 251

页数：10

共 59 条

[1] Laser-structured bacterial nanocellulose hydrogels support ingrowth and differentiation of chondrocytes and show potential as cartilage implants [J].