Novel Real-Time PCR Assays Using TaqMan Minor Groove Binder Probes for Identification of Fecal Carriage of Streptococcus bovis/Streptococcus equinus Complex from Rectal Swab Specimens

被引：16

作者：

Markus Lopes, Paulo Guilherme ^{[1
]}

Cantarelli, Vlademir Vicente ^{[2
]}

Agnes, Grasiela ^{[1
]}

Costabeber, Ane Micheli ^{[1
]}

d'Azevedo, Pedro Alves ^{[1
]}

机构：

[1] Hlth Sci Fed Univ Porto Alegre UFCSPA, Porto Alegre, RS, Brazil

[2] FEEVALE Univ, Novo Hamburgo, RS, Brazil

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 03期

关键词：

SUBSP NOV; BOVIS; GALLOLYTICUS; ASSOCIATION; COLON; INFECTION; CARCINOMA; POLYPS; CANCER; GENE;

D O I：

10.1128/JCM.03253-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time PCR based on the recN and gyrB genes was developed to detect four Streptococcus bovis/Streptococcus equinus complex (SBEC) subspecies from rectal swab specimens. The overall prevalence was 35.2%: Streptococcus gallolyticus subsp. gallolyticus (11.1%), S. gallolyticus subsp. pasteurianus (13%), Streptococcus infantarius subsp. coli (20.4%), and S. infantarius subsp. infantarius (11.1%). To conclude, these real-time PCR assays provide a reliable molecular method to detect SBEC pathogenic subspecies from rectal swab specimens.

引用

页码：974 / 976

页数：3

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