Glass bead cultivation of fungi: Combining the best of liquid and agar media

被引：17

作者：

Droce, Aida ^{[1
,2
]}

Sorensen, Jens Laurids ^{[1
,2
]}

Giese, Henriette ^{[1
]}

Sondergaard, Teis Esben ^{[1
,2
]}

机构：

[1] Aalborg Univ, Dept Biotechnol Chem & Environm Engn, DK-9000 Aalborg, Denmark

[2] Aarhus Univ, Fac Sci & Technol, Dept Anim Sci, DK-8830 Tjele, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 94卷 / 03期

关键词：

Fungal RNA extraction; Cultivation methods; Secondary metabolite production; Fusarium; Carbon metabolism; SOLID-STATE;

D O I：

10.1016/j.mimet.2013.07.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of bioactive compounds and enzymes from filamentous fungi is highly dependent on cultivation conditions. Here we present an easy way to cultivate filamentous fungi on glass beads that allow complete control of nutrient supply. Secondary metabolite production in Fusarium graminearum and Fusarium solani cultivated on agar plates, in shaking liquid culture or on glass beads was compared. Agar plate culture and glass bead cultivation yielded comparable results while liquid culture had lower production of secondary metabolites. RNA extraction from glass beads and liquid cultures was easier than from agar plates and the quality was superior. The system allows simple control of nutrient availability throughout fungal cultivation. This combined with the ease of extraction of nucleic acids and metabolites makes the system highly suitable for the study of gene regulation in response to specific nutrient factors. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：343 / 346

页数：4

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