A Chemo-Enzymatic Approach for Site-Specific Modification of the RNA Cap

被引：93
|
作者：
Schulz, Daniela [1 ]
Holstein, Josephin Marie [1 ]
Rentmeister, Andrea [1 ]
机构：
[1] Univ Hamburg, Dept Chem, Inst Biochem & Mol Biol, D-20146 Hamburg, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 30期
关键词：
AdoMet analogues; click chemistry; mRNA; trimethylguanosinesynthase; GIARDIA-LAMBLIA; SMALL NUCLEAR; METHYLTRANSFERASE; DNA; CHEMISTRY; APPARATUS; YEAST;
D O I：
10.1002/anie.201302874
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Capped and gowned: A two-step approach can be used to site-specifically modify the 5′-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m7G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol-ene or CuAAC click chemistry (see scheme). Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：7874 / 7878
页数：5
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