Miniaturized probe for femtosecond laser microsurgery and two-photon imaging

被引：106

作者：

Hoy, Christopher L. ^{[1
]}

Durr, Nicholas J. ^{[2
]}

Chen, Pengyuan ^{[1
]}

Piyawattanametha, Wibool ^{[3
]}

Ra, Hyejun ^{[3
]}

Solgaard, Olav ^{[3
]}

Ben-Yakar, Adela ^{[1
,2
]}

机构：

[1] Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[3] Stanford Univ, Dept Elect Engn, Stanford, CA USA

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 13期

关键词：

D O I：

10.1364/OE.16.009996

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Combined two-photon fluorescence microscopy and femtosecond laser microsurgery has many potential biomedical applications as a powerful "seek-and-treat" tool. Towards developing such a tool, we demonstrate a miniaturized probe which combines these techniques in a compact housing. The device is 10 x 15 x 40 mm(3) in size and uses an air-core photonic crystal fiber to deliver femtosecond laser pulses at 80 MHz repetition rate for imaging and 1 kHz for microsurgery. A fast two-axis microelectromechanical system scanning mirror is driven at resonance to produce Lissajous beam scanning at 10 frames per second. Field of view is 310 mu m in diameter and the lateral and axial resolutions are 1.64 mu m and 16.4 mu m, respectively. Combined imaging and microsurgery is demonstrated using live cancer cells. (c) 2008 Optical Society of America.

引用

页码：9996 / 10005

页数：10

共 41 条

[31]

Shen Nan, 2005, Mech Chem Biosyst, V2, P17

[32] Two-photon excitation fluorescence microscopy [J].

So, PTC ;

Dong, CY ;

Masters, BR ;

Berland, KM .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 :399-429

[33] Two-photon imaging to a depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier [J].

Theer, P ;

Hasan, MT ;

Denk, W .

OPTICS LETTERS, 2003, 28 (12) :1022-1024

[34] On the fundamental imaging-depth limit in two-photon microscopy [J].

Theer, Patrick ;

Denk, Winfried .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2006, 23 (12) :3139-3149

[35] Cell biology -: Targeted transfection by femtosecond laser [J].

Tirlapur, UK ;

König, K .

NATURE, 2002, 418 (6895) :290-291

[36] Spot size, depth-of-focus, and diffraction ring intensity formulas for truncated Gaussian beams [J].

Urey, H .

APPLIED OPTICS, 2004, 43 (03) :620-625

[37] Mechanisms of femtosecond laser nanosurgery of cells and tissues [J].

Vogel, A ;

Noack, J ;

Hüttman, G ;

Paltauf, G .

APPLIED PHYSICS B-LASERS AND OPTICS, 2005, 81 (08) :1015-1047

[38] In vitro and in vivo two-photon luminescence imaging of single gold nanorods [J].

Wang, HF ;

Huff, TB ;

Zweifel, DA ;

He, W ;

Low, PS ;

Wei, A ;

Cheng, JX .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (44) :15752-15756

[39] Neurosurgery - Functional regeneration after laser axotomy [J].

Yanik, MF ;

Cinar, H ;

Cinar, HN ;

Chisholm, AD ;

Jin, YS ;

Ben-Yakar, A .

NATURE, 2004, 432 (7019) :822-822

[40] Femtosecond infrared laser -: An efficient and safe in vivo gene delivery system for prolonged expression [J].

Zeira, E ;

Manevitch, A ;

Khatchatouriants, A ;

Pappo, O ;

Hyam, E ;

Darash-Yahana, M ;

Tavor, E ;

Honigman, A ;

Lewis, A ;

Galun, E .

MOLECULAR THERAPY, 2003, 8 (02) :342-350

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