Miniaturized probe for femtosecond laser microsurgery and two-photon imaging

被引：106

作者：

Hoy, Christopher L. ^{[1
]}

Durr, Nicholas J. ^{[2
]}

Chen, Pengyuan ^{[1
]}

Piyawattanametha, Wibool ^{[3
]}

Ra, Hyejun ^{[3
]}

Solgaard, Olav ^{[3
]}

Ben-Yakar, Adela ^{[1
,2
]}

机构：

[1] Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[3] Stanford Univ, Dept Elect Engn, Stanford, CA USA

来源：

OPTICS EXPRESS | 2008年 / 16卷 / 13期

关键词：

D O I：

10.1364/OE.16.009996

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Combined two-photon fluorescence microscopy and femtosecond laser microsurgery has many potential biomedical applications as a powerful "seek-and-treat" tool. Towards developing such a tool, we demonstrate a miniaturized probe which combines these techniques in a compact housing. The device is 10 x 15 x 40 mm(3) in size and uses an air-core photonic crystal fiber to deliver femtosecond laser pulses at 80 MHz repetition rate for imaging and 1 kHz for microsurgery. A fast two-axis microelectromechanical system scanning mirror is driven at resonance to produce Lissajous beam scanning at 10 frames per second. Field of view is 310 mu m in diameter and the lateral and axial resolutions are 1.64 mu m and 16.4 mu m, respectively. Combined imaging and microsurgery is demonstrated using live cancer cells. (c) 2008 Optical Society of America.

引用

页码：9996 / 10005

页数：10

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