Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells

被引：27

作者：

Cervelli, Manuela ^{[1
]}

Fratini, Emiliano ^{[1
,2
]}

Amendola, Roberto ^{[2
]}

Bianchi, Marzia ^{[1
]}

Signori, Emanuela ^{[3
]}

Ferraro, Elisabetta ^{[4
]}

Lisi, Antonella ^{[3
]}

Federico, Rodolfo ^{[1
]}

Marcocci, Lucia ^{[5
]}

Mariottini, Paolo ^{[1
]}

机构：

[1] Univ Roma Tre, Dipartimento Biol, I-00146 Rome, Italy

[2] CR Casaccia, ENEA, Dipartimento BAS BiotecMed, Rome, Italy

[3] CNR, Ist Neurobiol & Med Mol, Rome, Italy

[4] IRCCS Fdn Santa Lucia, Lab Mol Neuroembryol, I-00143 Rome, Italy

[5] Univ Roma La Sapienza, Dipartimento Sci Biochim A Rossi Fanelli, Rome, Italy

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2009年 / 41卷 / 04期

关键词：

Polyamines; Spermine oxidase; C2C12 muscle cells; Cell differentiation; OXIDATIVE STRESS; GROWTH-FACTOR; MUSCLE DIFFERENTIATION; NUCLEAR-LOCALIZATION; APOPTOSIS; IDENTIFICATION; EXPRESSION; POLYAMINES; INDUCTION; MEMBRANE;

D O I：

10.1016/j.biocel.2008.09.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H2O2, spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mouse myoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of the SMO alpha and SMO mu splice variants is not involved in the differentiation processes. Nuclear localization of only the SMO mu protein was confirmed. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：934 / 944

页数：11

共 35 条

[1] Direct oxidative DNA damage, apoptosis and radio sensitivity by spermine oxidase activities in mouse neuroblastoma cells [J].