Presence of a novel subset of NKT cells bearing an invariant Vα19.1-Jα26 TCR α chain

被引：33

作者：

Shimamura, M ^{[1
]}

Huang, YY ^{[1
]}

机构：

[1] Mitsubishi Kagaku Inst Life Sci, Lab Dev Immunol, Machida, Tokyo 1948511, Japan

来源：

FEBS LETTERS | 2002年 / 516卷 / 1-3期

关键词：

natural killer T cell; invariant TCR alpha chain; V-J junction; hybridoma; interleukin-4; secretion;

D O I：

10.1016/S0014-5793(02)02509-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CD1d-deficient (CD1d(-/-)) mouse lymphocytes were analyzed to classify the natural killer T (NKT) cells without reactivity to CD1d. The cells bearing a Valpha19.1-Jalpha26 (AV19-AJ33) invariant TCR alpha chain, originally found in the peripheral blood lymphocytes, were demonstrated to be abundant in the NK1.1(+) but not NK1.1(-) T cell population isolated from CD1d(-/-) mice. Moreover, more than half (11121) of the hybrid cell lines established from CD1d(-/-) NKT cells expressed the Valpha9.1-Jalpha26 invariant TCR alpha chain. The expression of the invariant Valpha19.1-Jalpha26 mRNA was absent in beta2-microglobulin-deficient mice. Collectively, the present findings suggest the presence of a second NKT cell repertoire characterized by an invariant TCR cc chain (Valpha19.1-Jalpha26) that is selected by an MTHC class I-like molecule other than CD1d. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：97 / 100

页数：4