Micropropagation of Prunus mume

被引：44
作者：
Harada, H
Murai, Y
机构：
[1] Faculty of Agriculture, Shizuoka University, Shizuoka
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 1996年 / 46卷 / 03期
关键词：
fructose; in vitro; rooting; shoot proliferation; sorbitol;
D O I：
10.1007/BF02307104
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Shoot proliferation from axillary buds of Prunus mume Sieb. et Zucc. was obtained on Woody Plant Medium (WPM) supplemented with 1 to 5 mu M benzyladenine, 3% sorbitol and solidified with 0.5 to 0.7% agar. Effects of different carbon sources on shoot proliferation were examined. Glucose provided better shoot proliferation than sucrose, sorbitol and fructose. In the presence of sucrose, leaf chlorosis occurred and shoots gradually declined. Best rooting percentage was obtained on WPM supplemented with 1 mu M naphthaleneacetic acid. Rooted plantlets were acclimatized under intermittent mist. However, survival rate was relatively low (20 to 30%).
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页码：265 / 267
页数：3
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