Bacteroidetes use thousands of enzyme combinations to break down glycans

被引：317

作者：

Lapebie, Pascal ^{[1
,2
]}

Lombard, Vincent ^{[1
,2
]}

Drula, Elodie ^{[1
,2
]}

Terrapon, Nicolas ^{[1
,2
]}

Henrissat, Bernard ^{[1
,2
,3
]}

机构：

[1] Inst Natl Agron INRA, CNRS, AFMB, UMR7257,USC 1408, F-13288 Marseille 9, France

[2] Aix Marseille Univ, F-13288 Marseille 9, France

[3] King Abdulaziz Univ, Dept Biol Sci, Jeddah, Saudi Arabia

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

欧洲研究理事会;

关键词：

HUMAN GUT MICROBIOTA; HYDROLASE FAMILY; DATABASE; CLASSIFICATION; NANOMACHINES; SUBFAMILIES; METABOLISM; CATABOLISM; MEMBERS;

D O I：

10.1038/s41467-019-10068-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Unlike proteins, glycan chains are not directly encoded by DNA, but by the specificity of the enzymes that assemble them. Theoretical calculations have proposed an astronomical number of possible isomers (>10(12) hexasaccharides) but the actual diversity of glycan structures in nature is not known. Bacteria of the Bacteroidetes phylum are considered primary degraders of polysaccharides and they are found in all ecosystems investigated. In Bacteroidetes genomes, carbohydrate-degrading enzymes (CAZymes) are arranged in gene clusters termed polysaccharide utilization loci (PULs). The depolymerization of a given complex glycan by Bacteroidetes PULs requires bespoke enzymes; conversely, the enzyme composition in PULs can provide information on the structure of the targeted glycans. Here we group the 13,537 PULs encoded by 964 Bacteroidetes genomes according to their CAZyme composition. We find that collectively Bacteroidetes have elaborated a few thousand enzyme combinations for glycan breakdown, suggesting a global estimate of diversity of glycan structures much smaller than the theoretical one.

引用

页数：7

共 51 条

[1] Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family [J].