Sensitive indirect spectrophotometric determination of isoniazid

被引：54

作者：

Safavi, A ^{[1
]}

Karimi, MA ^{[1
]}

Nezhad, MRH ^{[1
]}

Kamali, R ^{[1
]}

Saghir, N ^{[1
]}

机构：

[1] Shiraz Univ, Fac Sci, Dept Chem, Shiraz 71454, Iran

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2004年 / 60卷 / 04期

关键词：

isoniazid; spectrophotometric determination;

D O I：

10.1016/S1386-1425(03)00288-9

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A simple, rapid, sensitive and accurate indirect spectrophotometric method for the microdetermination of isoniazid (INH) in pure form and pharmaceutical formulations is developed. The procedure is based on the reaction of copper(II) with isoniazid in the presence of neocuproine (NC). In the presence of neocuproine, copper(II) is reduced easily by isoniazid to a Cu(I)-neocuproine complex, which shows an absorption maximum at 454 nm. By measuring the absorbance of the complex at this wavelength, isoniazid can be determined in the range 0.3-3.5 mug ml(-1). This method was applied to the determination of isoniazid in pharmaceutical formulation and enabled the determination of the isoniazid in microgram quantities (0.3-3.5 mug ml(-1)). The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official method and demonstrated good accuracy and precision. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：765 / 769

页数：5

共 17 条

[1] Sensitive liquid chromatographic technique to measure isoniazid in alveolar cells, bronchoalveolar lavage and plasma in HIV-infected patients [J].